| 鬼箭羽总黄酮含量测定方法的建立 |
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| 引用本文： | 党晓芳,曹飒丽,祁娟娟,张慧,张晋,付京,陈杨,倪健.鬼箭羽总黄酮含量测定方法的建立[J].中国实验方剂学杂志,2014,20(9):96-98. |
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| 作者姓名： | 党晓芳 曹飒丽 祁娟娟 张慧 张晋 付京 陈杨 倪健 |
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| 作者单位： | 北京中医药大学中药学院, 北京 100102;北京中医药大学中药学院, 北京 100102;北京中医药大学中药学院, 北京 100102;北京中医药大学中药学院, 北京 100102;北京中医药大学中药学院, 北京 100102;北京中医药大学中药学院, 北京 100102;北京中医药大学中药学院, 北京 100102;北京中医药大学中药学院, 北京 100102 |
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| 基金项目： | 北京中医药大学复方中药制药创新团队项目（2011-CX70-13） |
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| 摘 要： | 目的：建立鬼箭羽总黄酮的含量测定方法并筛选其提取方法。方法：以芦丁为对照品，通过单因素试验优选显色方法和显色条件，建立总黄酮含量测定的方法学考察并优选其提取方法。结果：采用盐酸-镁粉反应比色法，芦丁在0.020~0.104 mg与吸光度呈良好线性关系（r=0.997），平均加样回收率99.424%（RSD 2.220%）。结论：盐酸-镁粉显色法具有精密度高、重复性好等优势，适用于鬼箭羽总黄酮的定量分析。鬼箭羽总黄酮的提取宜采用回流法。
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| 关 键 词： | 鬼箭羽 总黄酮 显色条件 单因素试验 方法学考察 提取方式 |
| 收稿时间： | 2013/8/29 0:00:00 |
Optimization of Determination for Total Flavonoids in Euonymus alatus |
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| DANG Xiao-fang,CAO Sa-li,QI Juan-juan,ZHANG Hui,ZHANG Jin,FU Jing,CHEN Yang and NI Jian.Optimization of Determination for Total Flavonoids in Euonymus alatus[J].China Journal of Experimental Traditional Medical Formulae,2014,20(9):96-98. |
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| Authors: | DANG Xiao-fang CAO Sa-li QI Juan-juan ZHANG Hui ZHANG Jin FU Jing CHEN Yang and NI Jian |
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| Affiliation: | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China |
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| Abstract: | Objective: To establish a method for determination of the content of total flavonoids in Euonymus alatus. Method: Selecting rutin as a reference substance, single factor tests were adopted to optimize colorimetry methods and conditions, determination of the content of total flavonoids was established and optimized its extraction method. Result: Hydrochloric acid-Mg reaction assay was adopted, rutin had a liner relationship with absorbance in the range of 0.021-0.104 mg (r=0.997), average recovery was 99.424% with RSD of 2.220%. Conclusion: HCl-Mg colorimetry method appeared to be an accurate, reliable and repeatable system for determination of total flavonoids in E. alatus.Total flavonoids in E.alatus should employ reflux extraction method. |
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| Keywords: | Euonymus alatus total flavonoids colorimetry conditons single factor test methodological study extraction method |
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