北柴胡正丁醇部位保肝作用及其化学成分特征初步研究

目的:研究北柴胡正丁醇萃取部位对小鼠急性肝损伤的保护作用并对其化学成分特征进行初步研究.方法:采用腹腔注射四氯化碳诱导小鼠急性肝损伤,以柴胡正丁醇萃取部位高、中、低剂量(20,10,4 g·kg-1)同时预防给药7d,检测空白组、模型组、对照组、不同剂量柴胡正丁醇萃取部位组小鼠血清丙氨酸转氨酶(ALT),天冬氨酸转氨酶(AST)活性,同时测定肝组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性;建立并分析北柴胡正丁醇萃取部位HPLC色谱图.结果:北柴胡正丁醇萃取部位是以柴胡皂苷a和柴胡皂苷d为主要成分的柴胡总皂苷,可有效抑制造模小鼠血清中ALT,AST的升高(P＜0.05),同时可提高造模小鼠肝组织中SOD的活力,降低肝组织中MDA的含量(P＜0.05).结论:北柴胡正丁醇萃取部位对于小鼠急性肝损伤具有保护作用,其化学成分以柴胡皂苷类为主.

Liver Protective Effect and Chemical Constituent Features of n-butanol Extraction from Bupleurum chinense

Objective:To study the protective effect of n-butanol extraction from Bupleurum chinense on acute liver injury in mice induced by carbon tetrachloride(CCl₄) and to have a preliminary study on the features of its chemical constituent. Method:Preventive administration of total saikosaponin from B. chinense (high dose, middle dose, low dose) on acute liver injury mice induced by CCl₄ (ig) lasted for 7 d, then detect the serum AST and ALT activity and liver tissue MDA content and SOD activity in all groups (blank group, model group, control group and saikosaponins groups), meanwhile construct determination and analysis of HPLC chromatogram of n-butanol extraction of B. chinense were carried out. Result:Total saikosaponin which mainly contained saikosaponin a(SSa) and saikosaponin d(SSd) was the main constituent of n-butanol extraction of B. chinense and it could significantly decrease ALT and AST activity in serum(P<0.05)and meanwhile it could obviously enhance the activity of SOD and reduce the contents of MDA in liver(P<0.05). Conclusion:The n-butanol extraction of B.chinense has protective effects on acute liver injury induced by CCl₄ in mice and its main constituent was total saikosaponin.