| 响应面法优选铁皮石斛总多糖脱蛋白工艺 |
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| 引用本文： | 严婧,夏伯候,章莹,黄杰,谭茜,肖榕,廖端芳,吴萍.响应面法优选铁皮石斛总多糖脱蛋白工艺[J].中国实验方剂学杂志,2016,22(17):19-22. |
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| 作者姓名： | 严婧 夏伯候 章莹 黄杰 谭茜 肖榕 廖端芳 吴萍 |
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| 作者单位： | 湖南中医药大学 干细胞中药调控与应用研究室, 湖南省中药不良成分快速检测及脱除工程 技术研究中心, 中药有毒物质防控技术湖南省工程实验室, 长沙 410208,湖南中医药大学 干细胞中药调控与应用研究室, 湖南省中药不良成分快速检测及脱除工程 技术研究中心, 中药有毒物质防控技术湖南省工程实验室, 长沙 410208,湖南中医药大学 干细胞中药调控与应用研究室, 湖南省中药不良成分快速检测及脱除工程 技术研究中心, 中药有毒物质防控技术湖南省工程实验室, 长沙 410208,湖南中医药大学 干细胞中药调控与应用研究室, 湖南省中药不良成分快速检测及脱除工程 技术研究中心, 中药有毒物质防控技术湖南省工程实验室, 长沙 410208,湖南中医药大学 干细胞中药调控与应用研究室, 湖南省中药不良成分快速检测及脱除工程 技术研究中心, 中药有毒物质防控技术湖南省工程实验室, 长沙 410208,湖南中医药大学 干细胞中药调控与应用研究室, 湖南省中药不良成分快速检测及脱除工程 技术研究中心, 中药有毒物质防控技术湖南省工程实验室, 长沙 410208,湖南中医药大学 干细胞中药调控与应用研究室, 湖南省中药不良成分快速检测及脱除工程 技术研究中心, 中药有毒物质防控技术湖南省工程实验室, 长沙 410208,湖南中医药大学 干细胞中药调控与应用研究室, 湖南省中药不良成分快速检测及脱除工程 技术研究中心, 中药有毒物质防控技术湖南省工程实验室, 长沙 410208 |
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| 基金项目： | 湖南省教育厅重点项目（14A109）；湖南省科技厅发改委项目（湘发改高技[2013]1199号）；湖南省科技厅科研条件创新专项（2012TT2012）；湖南中医药大学校级课题（99820001134） |
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| 摘 要： | 目的:优选铁皮石斛总多糖的胰蛋白酶联合Sevage法脱蛋白工艺,为该有效部位的深度开发和综合利用提供参考。方法:以总多糖保存率和蛋白脱除率为评价指标,通过单因素试验比较Sevage法、木瓜蛋白酶法、胰蛋白酶法和胰蛋白酶联合Sevage法;以蛋白脱除率为指标,酶解时间、酶用量、酶解温度和粗多糖溶液与Sevage试剂体积比为考察因素,利用BoxBehnken响应面法优化条件。结果:胰蛋白酶联合Sevage法具有较高的总多糖保存率及蛋白脱除率;最佳工艺条件为酶解时间1.5 h,酶用量0.21 g·m L-1,酶解温度63.25℃,总多糖溶液-Sevage试剂(4.27∶1);蛋白脱除率(87.15±7.93)%,总多糖保存率(81.32±8.54)%。结论:不同方法对铁皮石斛总多糖的脱蛋白效果有明显差异,胰蛋白酶联用Sevage法的效果最佳。
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| 关 键 词： | 铁皮石斛 总多糖 脱蛋白 Sevage试剂 保留率 |
| 收稿时间： | 2015/10/19 0:00:00 |
Optimization of Deproteinization Technology of Total Polysaccharides from Dendrobii Officinalis Caulis by Response Surface Methodology |
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| YAN Jing,XIA Bo-hou,ZHANG Ying,HUANG Jie,TAN Qian,XIAO Rong,LIAO Duan-fang and WU Ping.Optimization of Deproteinization Technology of Total Polysaccharides from Dendrobii Officinalis Caulis by Response Surface Methodology[J].China Journal of Experimental Traditional Medical Formulae,2016,22(17):19-22. |
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| Authors: | YAN Jing XIA Bo-hou ZHANG Ying HUANG Jie TAN Qian XIAO Rong LIAO Duan-fang and WU Ping |
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| Affiliation: | Hunan Engineering Laboratory for Prevention and Control Technology of Toxic Substances of Chinese Medicine, Hunan Research Center of Engineering Technology for Rapid Test and Removal of Undesirable Components of Chinese Medicine, Stem Cell Laboratory of Regulation and Application of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China,Hunan Engineering Laboratory for Prevention and Control Technology of Toxic Substances of Chinese Medicine, Hunan Research Center of Engineering Technology for Rapid Test and Removal of Undesirable Components of Chinese Medicine, Stem Cell Laboratory of Regulation and Application of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China,Hunan Engineering Laboratory for Prevention and Control Technology of Toxic Substances of Chinese Medicine, Hunan Research Center of Engineering Technology for Rapid Test and Removal of Undesirable Components of Chinese Medicine, Stem Cell Laboratory of Regulation and Application of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China,Hunan Engineering Laboratory for Prevention and Control Technology of Toxic Substances of Chinese Medicine, Hunan Research Center of Engineering Technology for Rapid Test and Removal of Undesirable Components of Chinese Medicine, Stem Cell Laboratory of Regulation and Application of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China,Hunan Engineering Laboratory for Prevention and Control Technology of Toxic Substances of Chinese Medicine, Hunan Research Center of Engineering Technology for Rapid Test and Removal of Undesirable Components of Chinese Medicine, Stem Cell Laboratory of Regulation and Application of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China,Hunan Engineering Laboratory for Prevention and Control Technology of Toxic Substances of Chinese Medicine, Hunan Research Center of Engineering Technology for Rapid Test and Removal of Undesirable Components of Chinese Medicine, Stem Cell Laboratory of Regulation and Application of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China,Hunan Engineering Laboratory for Prevention and Control Technology of Toxic Substances of Chinese Medicine, Hunan Research Center of Engineering Technology for Rapid Test and Removal of Undesirable Components of Chinese Medicine, Stem Cell Laboratory of Regulation and Application of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China and Hunan Engineering Laboratory for Prevention and Control Technology of Toxic Substances of Chinese Medicine, Hunan Research Center of Engineering Technology for Rapid Test and Removal of Undesirable Components of Chinese Medicine, Stem Cell Laboratory of Regulation and Application of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China |
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| Abstract: | |
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| Keywords: | Dendrobii Officinalis Caulis total polysaccharides deproteinization Sevage reagent retention rate |
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