芪蛭皱肺颗粒对慢性阻塞性肺疾病模型大鼠细胞因子的影响

目的观察芪蛭皱肺颗粒对慢性阻塞性肺疾病（COPD）大鼠模型血清、支气管肺泡灌洗液（BALF）中白细胞介素-8（IL-8）、肿瘤坏死因子-α（TNF-α）和内皮素-1（ET-1）变化的影响，探讨其阻抑COPD的机制。方法Wistar大鼠随机分为空白组、模型组、阳性对照组和芪蛭大、中、小剂量组，采用气管内滴注脂多糖（LPS）加烟熏法复制COPD大鼠模型，造模后第15日开始药物干预，第43日采用放射免疫法分别检测血清、BALF中IL-8、TNF-α、ET-1含量的变化。结果与空白组比较，模型大鼠血清、BALF中TNF-α、IL-8、ET-1的含量显著升高（P〈0．05）。与模型组比较，芪蛭大剂量组血清中TNF-α水平明显降低（P〈0．05），芪蛭大、小剂量组血清IL-8水平明显降低（P〈0．05），芪蛭中剂量组血清中ET-1含量显著降低（P〈0．05），芪蛭中剂量组BALF中TNF-α的水平明显降低（P〈0．05），芪蛭大、中、小剂量组BALF中IL-8的水平明显降低（P〈0．05），芪蛭大、中剂量组BALF中ET-1含量显著降低（P〈0．05）。结论芪蛭皱肺颗粒对COPD大鼠肺组织具有保护作用，调节IL-8、TNF-α、ET-1含量可能是其干预COPD发生、发展的机制之一。

Effect of Qizhi Zhoufei Granules on Cytokines of Chronic Obstructive Pulmonary Disease Model Rats

Objective To observe the effects of Qizhi Zhoufei Granules on IL-8, TNF-α and ET-1 in serum and bronchoalveolar lavage fluid （BALF） of chronic obstructive pulmonary disease （COPD） model rats, and to study its mechanism of preventing and treating COPD. Methods Wistar rats were randomly divided into blank group, model group, positive control group, Qizhi low-dose group, Qizhi medium-dose group and Qizhi high-dose group. The COPD model was established by smoking and infusing lipopolysaccharide （LPS）. After 15 days, the rats were given the drugs by orally taking once a day. On the 43rd day, the change of IL-8, TNF-a and ET-1 content in serum and BALF were measured by radio-immunity assay. Results Compared with the blank group, the level of IL-8, TNF-α and ET-1 in serum and BALF were considerably raised in model group （P 〈0.05）. Whereas compared with the model group, the level of TNF-α in serum of Qizhi high-dose group was remarkably lowered （P〈0.05）, the level of IL-8 in serum of Qizhi low-dose group and Qizhi high-dose group was remarkably lowered （P〈0.05）, the level of TNF-a in BALF of Qizhi medium-dose group was remarkably lowered （P〈0.05）, the level of IL-8 in BALF of Qizhi low-, medium- and high-dose group was remarkably lowered （P〈0.05）, and the level of ET-1 in BALF of Qizhi high-dose group and Qizhi medium-dose group was remarkably lowered （P 〈0.05）. Conclusion Qizhi Zhoufei Granules have protective effect on lung tissue of COPD rats, and can adjust the content of IL-8, TNF-α and ET-1, which may be one of its mechanisms of intervening occurrence and development of COPD.