预发酵-双相联合酸水解法萃取薯蓣皂素的初步研究简

 目的 初步建立预发酵-双相联合酸水解法从黄姜药材中萃取薯蓣皂素的工艺。方法 采用正交实验设计法, 以薯蓣皂素的得率为考察指标, 分别优化预发酵和双相联合酸水解法的最佳条件, 并与两类传统提取方法进行比较。结果 将黄姜药材粉末在500 r·min^-1搅拌下于30 ℃预发酵72 h后, 加入浓硫酸和甲醇使它们的终浓度均为10%, 再加入石油醚形成双相体系, 并于120 ℃油浴中加热回流萃取4 h, 薯蓣皂素的平均得率为4.28%;而两类传统水解方法的得率则分别为3.01%和3.28%。结论 利用预发酵-双相联合酸水解法从黄姜药材中萃取薯蓣皂素简便易行, 并且提取率明显高于其他方法。

Fermentation Pretreatment Followed by Two-Phase Combination Acid Hydrolysis for the Extraction of Diosgenin

OBJECTIVE To preliminarily establish the technology of fermentation pretreatment followed by two-phase combination acid hydrolysis for the extraction of diosgenin from the dry tubers of Dioscorea zingiberensis C. H. Wright. METHODS Orthogonal experiment design was used with the yield of diosgenin as the monitoring index to optimize the conditions for fermentation pretreatment and two-phase combination acid hydrolysis. Two types of conventional METHODS were compared with the newly developed method. RESULTS Fine powder of the tubers of Dioscorea zingiberensis C. H. Wright was mixed with water and fermented at 30 ℃ for 72 h under stirring at 500 r·min^-1. After that, sulfuric acid and methanol were added into the suspension to reach their final concentration of 10%, and petroleum ether was added to form a two-phase immiscible reaction system which was extracted in oil bath at 120 ℃ for 4 h. As a consequence, the average yield of diosgenin achieved 4.28%, significantly higher than the 3.01% and 3.28% of the two conventional methods. CONCLUSION This newly developed method of fermentation pretreatment followed by two-phase combination acid hydrolysis is proved to be more convenient than the traditional METHODS with a higher yield.