|
|||||
|
|
| 125I标记抗体法优化免疫磁性微球的构建条件 | |
| 引用本文： | 康继超,沙木屯布卡,贺师鹏,谢蜀生,魏树礼.125I标记抗体法优化免疫磁性微球的构建条件[J].中国药学杂志,1998,33(3):148-151. |
| 作者姓名： | 康继超 沙木屯布卡 贺师鹏 谢蜀生 魏树礼 |
| 作者单位： | 1. 北京 100083 北京医科大学药剂教研室 2. 北京医科大学免疫药理研究室 3. 北京医科大学同位素室 |
| 摘 要： | 目的：研究磁性微球与单克隆抗体结合的条件，以构建具有良好性能的免疫磁性微球。方法：以同位素标记的单克隆抗体和微球的结合率为指标。用均匀设计法研究影响免疫磁性微球构建的因素。结果：pH值、抗体加入量和反应时间是影响免疫磁性微球构建的主要因素。结论：通过控制条件，可以构建具有良好性能的免疫微球。 |
| 关 键 词： | 免疫磁性微球 同位素标记抗体 均匀设计法 |
| 收稿时间： | 1997-02-24; |
Optimal construction of an immunomagnetic microspheres by 125 I labelled antibody methods |
|
| Kang Jichao.Optimal construction of an immunomagnetic microspheres by 125 I labelled antibody methods[J].Chinese Pharmaceutical Journal,1998,33(3):148-151. | |
| Authors: | Kang Jichao |
| Affiliation: | (Kang jc)，Santem Buka (Santem BK)，He Shipeng (He SP)，et a |
| Abstract: | OBJECTIVE: To study the factors affecting the binding of monoclonal antibodies to made magnetic microspheres in order to construct an immunomagnetic microspheres that have good properties. METHODS: What determines the binding percent of monoclonal antibodies to make magnetic beads is studied by uniform design methods, isotope marked antibody's binding percent as the criteria. RESULTS: The main factors affecting the construction of an immunomagnetic microspheres included pH, the amount of antibodies added, reaction time. CONCLUSION: By selecting the antibodies binding conditions, immunomagnetic microspheres which have good properties can be easily constructed. |
| Keywords: | immunomagnetic microspheres isotope marked antibodies uniform design methods |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《中国药学杂志》浏览原始摘要信息 | |
| 点击此处可从《中国药学杂志》下载全文 | |
