基于传统性状客观化分析的川贝母质量评价

目的对川贝母的外观性状进行客观量化,完善其质量评价方法。方法用游标卡尺测定高度和直径,在人眼对外观色泽观察的基础上用色差仪对粉末色泽进行客观量化,通过紫外分光光度法测定总生物碱的含量,对高度、直径、△E值与总生物碱含量测定结果进行统计学分析,确定外观性状与内在成分之间的相关程度。结果高度与总生物碱的含量呈极显著负相关(P<0.01)、直径与总生物碱的含量呈负相关,综合来看随着川贝母鳞茎的增大,总生物碱含量呈降低趋势。△E值与总生物碱含量之间的相关性不显著。结论由于总生物碱含量可以客观地反映川贝母的质量,本实验的结果为川贝母传统以"个小为佳"、"碎贝、破贝不可入药"的观点赋予了科学的依据,肯定了川贝母"不得水洗"的传统加工方法。不同规格的川贝母中以松贝为优。

Quality Evaluation of Fritillariae Cirrhosae Bulbus Based on Traditional Character Analysis

OBJECTIVE To improve the quality evaluation method of Fritillariae Cirrhosae Bulbus by the means of objective quantization of appearance character.METHODS The height and diameter were measured by a vernier caliper.The color of powder was determined by a colorimeter based on the human eye observation.The content of total alkaloids was measured by the ultraviolet spectroscopy.Some correlation analysis between the content of total alkaloids and height,diameter,and△E value were evaluated by a SPSS software,respectively.RESULTS There were a very significant negative correlation(P<0.01)between height and the total alkaloids,a negative correlation between diameter and the total alkaloids,and no significant correlation between△E value and the total alkaloids.CONCLUSION Because the content of total alkaloids could reflect the quality of Fritillariae Cirrhosae Bulbus objectively,these results of this study provide a scientific basis for the traditional ideas of"the smaller the best"and"the broken Fritillariae Cirrhosae Bulbus could not be used as medicine"and affirm the traditional processing technology that"should not be washed by water".Songbei was the best Fritillariae Cirrhosae Bulbus of them all.