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| 测定穿山龙中薯蓣皂苷元的前处理方法的改进 | |
| 引用本文： | 王俊,陈钧,杨克迪.测定穿山龙中薯蓣皂苷元的前处理方法的改进[J].中国药学杂志,2005,40(9):660-663. |
| 作者姓名： | 王俊 陈钧 杨克迪 |
| 作者单位： | 1. 江苏科技大学生物技术学院,江苏镇江 212018 2. 江苏大学生物与环境工程学院,江苏,镇江,212013 3. 广西大学化学化工学院,广西,南宁,530004 |
| 基金项目： | 江苏省高校自然科学基金 |
| 摘 要： | 目的研究一种测定穿山龙中薯蓣皂苷元时快速提取薯蓣皂苷元的新方法——水解原位萃取法。方法对影响提取率的重要因素进行了考察,以期建立最佳提取条件和适宜的纯化方法。结果样品用含约1.5 mol·L-1硫酸的70%异丙醇水溶液在沸水浴中加热回流4h,获得了比传统方法更高的提取率。结论水解原位萃取法简单、省时、准确。 |
| 关 键 词： | 薯蓣皂苷元 水解原位萃取法 穿山龙 高效液相色谱法 |
| 文章编号： | 1001-2494(2005)09-0660-04 |
| 收稿时间： | 2004-04-04; |
Study on the pretreatment of diosgenin in Dioscorea nipponica |
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| WANG Jun,CHEN Jun,YANG Ke-Di.Study on the pretreatment of diosgenin in Dioscorea nipponica[J].Chinese Pharmaceutical Journal,2005,40(9):660-663. | |
| Authors: | WANG Jun CHEN Jun YANG Ke-Di |
| Affiliation: | 1.College of Biotechnology，Jiangsu University of Science and Technology， Zhenjiang 212018，China；2.School of Biological and Environmental Engineering，Jiangsu University，Zhenjiang 212013，China； 3.College of Chemistry and Chemical Engineering， Guangxi University， Nanning 530004，China |
| Abstract: | OBJECTIVE To study a new extraction method for analyzing diosgenin in Dioscorea nipponica Makino.---extraction in combination hydrolysis in situ.METHODS The effective factors on the yield of diosgenin were evaluated in order to establish the optimum conditions for extraction in combination hydrolysis in situ and the suitable method for isolating diosgenin from the crude extracts. RESULTS Hydrolyses was conducted in 1.5 mol·L-1 sulfuric acid with 70% isopropanol at 100℃ for 4 h. The higher recovery of diosgenin was obtained by the extraction in combination hydrdysis in situ than that by traditional methods.CONCLUSION The method is simple, accurate and time saving. |
| Keywords: | diosgenin extraction in combination hydrolysis in situ Dioscorea nipponica Makino HPLC |
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