| 灵芝过氧化物同工酶和酯酶同工酶的研究 |
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| 引用本文： | 兰进,徐锦堂,王秋颖,贺秀霞.灵芝过氧化物同工酶和酯酶同工酶的研究[J].中国药学杂志,1998,33(12):714-716. |
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| 作者姓名： | 兰进 徐锦堂 王秋颖 贺秀霞 |
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| 作者单位： | 北京,100094,中国医学科学院-中国协和医科大学药用植物研究所 |
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| 摘 要： | 目的：探讨利用同工酶技术对灵芝不同菌株的分类鉴定方法。方法：应用聚丙烯酰胺凝胶电泳法，分析比较过氧化物同工酶和酯酶同工酶酶谱的变化。结果：灵芝5个菌株间存在相似的酶带和相近的Rf值，亲缘关系极其相近，紫芝与其它菌株间酶谱差异较大，亲缘关系相对较远。结论：利用酯酶同工酶对灵芝菌株及菌种进行分类鉴定是一种可靠的方法。
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| 关 键 词： | 灵芝 过氧化物同工酶 酯酶同工酶 电泳 |
| 收稿时间： | 1997-07-31; |
Electrophoretic studies on estease and peroxidase isozymes in Ganoderma sp. |
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| Lan Jin.Electrophoretic studies on estease and peroxidase isozymes in Ganoderma sp.[J].Chinese Pharmaceutical Journal,1998,33(12):714-716. |
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| Authors: | Lan Jin |
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| Affiliation: | (Lan J)，Xu Jintang(Xu JT)，Wang Qiuying(Wang QY)，et a |
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| Abstract: | OBJECTIVE:To establish a method of electrophoresis and isozymography to classify and identify Ganoderma.METHOD:The changes in isoenzyme pattern were characterized by polyacrylamide gel electrophoretic (PAGE) and compared. RESULTS:The five strains of Ganoderma had some similar bands with common hereditary characteristics and close relationship. While isoenzyme zymogram of G. sinense was different from other strains with a distant relation. CONCLUSION:Estease isozyme analyses is an effective way to classify strain of Ganoderma. |
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| Keywords: | Ganoderma sp peroxidase isozyme estease isozyme electrophoresis |
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