| 卷柏及垫状卷柏的HPLC指纹图谱及穗花杉双黄酮的含量测定研究 |
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| 引用本文： | 范慧佳,梅赞,杨杰,曹园,王强.卷柏及垫状卷柏的HPLC指纹图谱及穗花杉双黄酮的含量测定研究[J].现代中药研究与实践,2011(3):31-33. |
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| 作者姓名： | 范慧佳 梅赞 杨杰 曹园 王强 |
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| 作者单位： | [1]中国药科大学,江苏南京210009 [2]江苏省中医院,江苏南京210036 |
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| 基金项目： | 国家“重大新药创新”科技重大专项资助项目（NO：20092X09308-04） |
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| 摘 要： | 目的 建立卷柏及垫状卷柏的HPLC指纹图谱分析方法并进行穗花杉双黄酮的含量测定.方法 采用HPLC方法测定24批不同产地的卷柏及垫状卷柏药材,分析两者的指纹图谱特征及穗花杉双黄酮的含量.结果 以穗花杉双黄酮为主的不同的7个特征峰分别共同构成卷柏及垫状卷柏的HPLC指纹图谱概貌,卷柏及垫状卷柏中穗花杉双黄酮的含量分别为1...
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| 关 键 词： | 卷柏 垫状卷柏 HPLC 指纹图谱 穗花杉双黄酮 含量测定 |
Study on HPLC Fingerprint Chromatogram of Selaginella tamariscina and Selaginella pulvinate and Amentoflavone Determination |
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| FAN Hui-jia,MEI Zan,YANG Jie,CAO Yuan,WANG Qiang.Study on HPLC Fingerprint Chromatogram of Selaginella tamariscina and Selaginella pulvinate and Amentoflavone Determination[J].Research and Practice on Chinese Medicines,2011(3):31-33. |
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| Authors: | FAN Hui-jia MEI Zan YANG Jie CAO Yuan WANG Qiang |
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| Affiliation: | 1. China Pharmaceutical University, Nanjing 210009,China;2. Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing 210036,China) |
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| Abstract: | Objective To establish a method to analyze HPLC fingerprint chromatogram of Selaginella tamariscina and Selaginella pulvinate and to determine the content of amentoflavone.Methods HPLC was applied for establishment of fingerprints of Selaginell atamariscina and Selaginella pulvinate which from different origin to analyze their fingerprint characteristic and content of amentoflavone.Results The different characteristic seven peaks in the chromatogram consisted of the common pattern of Herba Selaginella tamariscina and Selaginlla pulvinate.Two important mutual fingerprint peaks were found in both of them,one of which is amentoflavone.The content of amentoflavone in Herba Selaginella tamariscina and Selaginella pulvinate was 1.77-3.82% and 0.45-0.85% separately.Conclusion The HPLC fingerprint chromatogram of Selaginella tamariscina and Selaginella pulvinate was similar,but their were very different in the content of amentoflavone. |
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| Keywords: | Selaginella tamariscina Selaginella pulvinate HPLC fingerprint amentoflavone assay |
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