| RP-HPLC测定天麻中天麻素的含量 |
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| 引用本文： | 王莉,徐青,肖红斌,梁鑫淼.RP-HPLC测定天麻中天麻素的含量[J].现代中药研究与实践,2004,18(Z1):43-44. |
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| 作者姓名： | 王莉 徐青 肖红斌 梁鑫淼 |
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| 作者单位： | 中国科学院大连化学物理研究所,辽宁,大连,116011 |
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| 基金项目： | 中国科学院知识创新工程重要方向性项目(KGCX2-SW-213) |
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| 摘 要： | 目的 建立RP-HPLC测定天麻中天麻素含量的分析方法。方法 优化药材的提取及色谱的分析方法得分离良好的色谱图,外标法定量。色谱柱:Hypersil ODS色谱柱(4.6×250 mm);流动相:甲醇-0.1％冰醋酸水溶液(5:95);检测波长:270 nm。结果 色谱图中12个峰均可用其进行准确定量,其中天麻素含量测定的线性范围为0.60~2.41μg(r=0.999 9),平均加样回收率为97.85％,RSD=1.17％(n=5)。结论 本方法简便,准确,重现性好,可用于天麻中天麻素的含量测定,同时也可用于其它水溶性成分的定量。
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| 关 键 词： | 天麻 天麻素 HPLC |
| 文章编号： | 1004-2199(2004)S0-0043-02 |
Determination of Gastrodin in Gastrodia eleta Bl.By RP-HPLC |
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| WANG Li,XU Qing,XIAO Hong-bin,LIANG Xin-miao.Determination of Gastrodin in Gastrodia eleta Bl.By RP-HPLC[J].Research and Practice on Chinese Medicines,2004,18(Z1):43-44. |
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| Authors: | WANG Li XU Qing XIAO Hong-bin LIANG Xin-miao |
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| Abstract: | Objective To constitute the analytical method for determination gastrodin in Gastrodia elata Bl. by RP-HPLC. Method Optimize the extracting and separate method to get the superior spectrum for quantilizing gastrodin by standard curve. HPLC analysis was carried out with C18 column and methanol-0. l%acetic acid (5 : 95) as the mobile phase. The detection wavelength was 270 nm. Result Twelve peaks can be used for quantity, in which gastrodin was determinated with 0. 60-2. 41 μg (r=0. 9999) as the linear range and 97. 85% (RSD=1. 17%, n = 5) as the average recovery. Conclusion The method is simple, reliable and good recurrence. It can be used to determine gastrodin in Gastrodia elata Bl. , and other water soluble constituents as well. |
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| Keywords: | Gastrodia elata Bl Gastrodin HPLC |
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