3种乳化体系乳膏中黄芩苷、苦参碱、甘草次酸和大黄素的体外经皮渗透的影响研究

研究烷基糖苷、司盘-吐温和A6/25 3种乳化体系对乳膏中黄芩苷、苦参碱、甘草次酸和大黄素的体外经皮渗透的影响。以改良的Franz扩散池和离体小鼠皮进行体外渗透实验,采用HPLC同时测定3种不同乳化体系乳膏中4种中药单体的累积透过量与皮肤滞留量,并考察不同中药成分在相同乳化体系乳膏及不同乳化体系乳膏对相同中药成分的累积透过量、皮肤滞留量及透皮吸收速率的影响。结果表明,不同中药成分在相同乳化体系的累积透过量及透皮吸收速率大小为苦参碱黄芩苷甘草次酸大黄素。不同乳化体系乳膏对相同中药成分的累积透过量及透皮吸收速率的影响除甘草次酸和大黄素无显著性差异外,司盘-吐温乳化体系的累积透过量及透皮吸收速率较大。各中药成分在3种乳化体系中累积透过量及透皮吸收速率规律具有一致性。而不同乳化体系对相同中药成分的累积透过量、皮肤滞留量及透皮吸收速率的影响并无一致性规律。

Effects of baicalin, matrine, glycyrrhetinic acid and emodin in three kinds of emulsifier cream system on transdermal absorption in vitro

To study effects of APG, Span-Tween and A6/25 emulsifier cream system on transdermal absorption in vitro of baicalin, matrine, glycyrrhetinic acid and emodin in emulsifier. Permeations studies were carried out in vitro with excised mice skin by improved Franz diffusion cells. The cumulative penetration amounts and the retention amounts of Chinese herbal medicinal ingredients in three kinds of emulsifier cream systems were determined by HPLC. The effects of different Chinese herbal medicinal ingredients in the same emulsifier system and the same herbal medicinal ingredients in different emulsifier systems on cumulative permeation amount, skin retention amount and permeation rate were investigated. According to the results, the order of different Chinese herbal medicinal ingredients in same kinds of emulsifier system by the cumulative permeation amount and the permeation rate were matrine> baicalin> glycyrrhetinic acid> emodin. With respect to the effect of different emulsifier systems on cumulative permeation amount and permeation rate of the same herbal medicinal ingredients, glycyrrhetinic acid and emodin showed no significant difference, Span-Tween emulsifier cream system had higher cumulative permeation amount and permeation rate. The cumulative permeation amount and the permeation rate of Chinese herbal medicinal ingredients in the three kinds of emulsifier cream systems had an identical regularity. However, the cumulative permeation amount, the skin retension amount and the permeation rate of the same herbal medicinal ingredients in different emulsifier systems had no regularity.