| 高效液相色谱法测定中药凌霄花中麦角甾苷、齐墩果酸和熊果酸含量 |
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| 引用本文： | 张桥,沈娟,柳于介,毕宇安,王振中,萧伟.高效液相色谱法测定中药凌霄花中麦角甾苷、齐墩果酸和熊果酸含量[J].中国中药杂志,2011,36(8):1043-1045. |
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| 作者姓名： | 张桥 沈娟 柳于介 毕宇安 王振中 萧伟 |
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| 作者单位： | 江苏康缘药业股份有限公司,江苏,连云港,222001;中药制药过程新技术国家重点实验室,江苏,连云港,222001;江苏省企业院士工作站,江苏,连云港,222001 |
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| 基金项目： | 国家"重大新药创制"科技重大专项(2009ZX09313-032) |
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| 摘 要： | 目的:建立中药凌霄花中麦角甾苷、齐墩果酸、熊果酸含量测定方法.方法:色谱柱Agilent ZORBAX EclipseXDB-18,流动相甲醇-0.1%磷酸梯度洗脱,检测波长:0-30 min为334nm,30～60 min为210nm.结果:麦角甾苷、齐墩果酸和熊果酸3成分浓度分别在0.025 9-0.258,0.100-1.00,0.104-1.04 g·L-1与峰面积呈良好线性关系,平均加样回收率依次为98.9%,99.3%,99.4%.结论:可同时测定麦角甾苷、齐墩果酸和熊果酸含量,可用于药材质量控制.
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| 关 键 词： | 凌霄花 麦角甾苷 齐墩果酸 熊果酸 含量测定 高效液相色谱法 |
| 收稿时间： | 2010/11/11 0:00:00 |
Simultaneous determination of acteoside, oleanolic acid and ursolic acid in flower of Campsis grandiflora by HPLC |
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| ZHANG Qiao,SHEN Juan,LIU Yujie,BI Yu''an,WANG Zhenzhong and XIAO Wei.Simultaneous determination of acteoside, oleanolic acid and ursolic acid in flower of Campsis grandiflora by HPLC[J].China Journal of Chinese Materia Medica,2011,36(8):1043-1045. |
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| Authors: | ZHANG Qiao SHEN Juan LIU Yujie BI Yu'an WANG Zhenzhong and XIAO Wei |
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| Affiliation: | Jiangsu Kanion Pharmaceutical Co.,Ltd., Lianyungang 222001, China;State Key Laboratory of Pharmaceutical Process New-tech for Chinese Medicine, Lianyungang 222001, China;Enterprises Academician Workstations in Jiangsu Province, Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co.,Ltd., Lianyungang 222001, China;State Key Laboratory of Pharmaceutical Process New-tech for Chinese Medicine, Lianyungang 222001, China;Enterprises Academician Workstations in Jiangsu Province, Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co.,Ltd., Lianyungang 222001, China;State Key Laboratory of Pharmaceutical Process New-tech for Chinese Medicine, Lianyungang 222001, China;Enterprises Academician Workstations in Jiangsu Province, Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co.,Ltd., Lianyungang 222001, China;State Key Laboratory of Pharmaceutical Process New-tech for Chinese Medicine, Lianyungang 222001, China;Enterprises Academician Workstations in Jiangsu Province, Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co.,Ltd., Lianyungang 222001, China;State Key Laboratory of Pharmaceutical Process New-tech for Chinese Medicine, Lianyungang 222001, China;Enterprises Academician Workstations in Jiangsu Province, Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co.,Ltd., Lianyungang 222001, China;State Key Laboratory of Pharmaceutical Process New-tech for Chinese Medicine, Lianyungang 222001, China;Enterprises Academician Workstations in Jiangsu Province, Lianyungang 222001, China |
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| Abstract: | Objective : To develop an HPLC method for the determination of acteoside, oleanolic acid and ursolic acid in flowers of Campsis grandiflora. Method : The analysis was carried out on an Agilent ZORBAX Eclipse XDB-18 colunm eluted with methanol and 0.1% phosphoric acid in gradient mode. The detection wavelength was set at 334 nm at 0-30 min and 210 nm at 30-60 min. Result : The peak areas and concentrations have a good linear relationship at 0.025 9-0.258 g·L-1 for acteoside, 0.100-1.00 g·L-1 for oleanolic acid and 0.104-1.04 g·L-1 for ursolic acid, respectively. The average recoveries were 98.9%, 99.3% and 99.40%, respectively. Conclusion : The method can determinate the concentration of acteoside, oleanolic acid and ursolic acid simultaneously. It can be used for the quality control of flower of C. grandiflora. |
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| Keywords: | flower of Campsis grandiflora acteoside oleanolic acid ursolic acid assay HPLC |
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