全蝎、蜈蚣对胶原诱导型关节炎大鼠小肠黏膜IL-2、IL-4、IL-10表达及关节损伤的影响

目的观察全蝎、蜈蚣对胶原诱导型关节炎(collagen-induced arthritis,CIA)大鼠小肠黏膜白细胞介素(interleukin,IL)-2(IL-2)、IL-4、IL-10表达及关节损伤的影响。方法 60只Wistar大鼠随机分成正常对照组(正常组)、模型组、全蝎、蜈蚣高、中、低剂量组和Ⅱ型胶原蛋白(typeⅡcollagen,CⅡ)治疗组(CⅡ组),采用CⅡ诱导的大鼠类风湿性关节炎模型,给药40天,肉眼观察大鼠关节损伤,测量大鼠关节容积,采用ELISA法检测小肠组织匀浆IL-2、IL-4、IL-10的表达水平。结果模型组大鼠关节损伤评分及左右后肢足爪容积自给药第23天后明显高于正常组(P<0.01),伴足趾及踝关节红肿变形,甚至影响行走。给药后第32天,4个治疗组关节损伤评分及足爪容积明显降低(P<0.05,P<0.01)。模型组大鼠小肠组织匀浆IL-2、IL-4、IL-10表达水平明显低于正常组(P<0.05,P<0.01),与模型组比较,仅全蝎、蜈蚣高剂量组及CⅡ组小肠组织匀浆IL-2、IL-4表达显著升高,全蝎、蜈蚣高、中剂量组及CⅡ组IL-10表达显著升高(P<0.05,P<0.01)。结论全蝎、蜈蚣可有效缓解胶原免疫性关节炎大鼠的关节损伤,其作用机制可能与小肠黏膜IL-2、IL-4、IL-10表达水平升高相关。

Effects of Scorpion and Centipede on IL-2,IL-4,and IL-10 in the Small Intestinal Mucosa and Joint Injury of Rats with Collagen Induced Arthritis

Objective To observe the effects of scorpion and centipede on interleukin(IL)-2,IL-4,IL-10 in the small intestinal mucosa and joint injury of rats with collagen induced arthritis(CIA).Methods Sixty Wistar rats were randomly divided into 6 groups:the normal control group,the model group,the low dose scorpion and centipede group,the middle dose scorpion and centipede group,the high dose scorpion and centipede group,and the type Ⅱ collagen treatment group.The joints’ volume was measured 40 days after type Ⅱ collagen(CⅡ) induced rheumatoid arthritis model was established.The joint injury was observed by naked eyes.The expression levels of IL-2,IL-4,and IL-10 in the small intestine tissue homogenate were detected by enzyme linked immunosorbent assay(ELISA).Results The joint injury score and volume of two hind limbs were obviously higher in the model group than in the normal control group since the 23rd day(P<0.01).Rats were accompanied with red,swollen,and deformed foot toes and ankle joints.Walking was even affected.Meanwhile,the joint injury score and volume of two hind limbs were obviously lowered by medicated with 0.4,0.2,0.1 g/kg scorpion and centipede,as well as CⅡ on the 32nd day after medication(P<0.05,P<0.01).The expression levels of IL-2,IL-4,and IL-10 in the small intestine tissue homogenate were obviously lower in the model group than in the normal control group(P<0.05,P<0.01).Compared with the model group,only the expression levels of IL-2 and IL-4 in the small intestine tissue homogenate of the high dose scorpion and centipede group and the type Ⅱ collagen treatment group significantly increased.The expression level of IL-10 significantly increased in the high and middle dose scorpion and centipede groups,as well as the type Ⅱ collagen treatment group,showing statistical difference(P<0.05,P<0.01).Conclusions Scorpion and centipede could effectively release the joint injury of rats with CIA.Its mechanism might be correlated with increased expression levels of IL-2,IL-4,and IL-10 in the small intestine mucosa.