小柴胡汤抗鸭乙肝病毒的实验研究

目的：通过体内抗鸭乙型肝炎病毒（ＤＨＢＶ）的实验研究，印证小柴胡汤治疗慢性乙型肝炎的独特疗效。揭示其扶正祛邪组方机理的合理性。方法：按人体公斤体重折算分为小柴湖汤２０、１５、５倍不同剂量组，根据所含药物不同分成小柴胡汤全方组、半方组和单味柴胡组，分别观察其抗ＤＨＢＶ的不同作用。并与抗病毒西药无环鸟苷（ＡＣＶ）进行比较。结果：小柴胡汤２０倍剂量组对ＤＨＢＶ的抑制作用最佳；小柴胡汤不同组别对ＤＨＢＶ均

Experimental study on inhibitory effect of xiaochaihu decoction on duck hepatitis B virus]

OBJECTIVE: To verify the therapeutic effect of Xiaochaihu decoction (XCHD) on chronic hepatitis B, and to prove the rationality of the TCM principle "strengthening the body resistance to eliminate pathogenic factors". METHODS: The inhibitory effects on DHBV of various doses of XCHD and its ingredients (grouped into whole recipe, partial recipe and single Bupleurum) were determined and compared, as well as compared with that of the blank control and of antiviral drug, acyclovir (ACV). RESULTS: Inhibitory effect was shown in all the treated groups. The treatment by whole recipe of XCHD with the dose 20 times that of clinical use showed the optimal effect, the difference in comparing with other treated groups was significant, P < 0.05. The effect of whole recipe was better than that of the partial recipe or single Bupleurum, P < 0.05. Moreover, the effect was rather persistent, no rebound phenomena was observed after withdrawal of medication, while in the ACV treated group, though a better effect conld be obeained, however, the DHBV returned to the level of before treatment after cessation of ACV treatment. CONCLUSION: The therapeutic effect of XCHD might be to supplment body resistance and remove the evil pathogen, strengthen or regular immune function, so XCHD is efficient in treating chronic hepatitis B.