人参皂苷Rb1对氧化型低密度脂蛋白诱导的人单核细胞源树突状细胞免疫成熟的影响

目的 研究中药单体人参皂苷Rb1(ginsenoside Rb1, GRb1)对氧化型低密度脂蛋白(oxidized low density lipoprotein, OX-LDL)诱导的人单核细胞源树突状细胞(dendritic cells, DCs)免疫成熟的影响。

Effect of Ginsenoside Rb1 on Immune Maturation of Human Monocyte-Derived Dendritic Cells Induced by Oxidized Low-Density Lipoprotein

Objective To examine the effect of ginsenoside Rb1（GRb1） on the immune maturation of monocyte-derived dendritic cells（DCs） induced by oxidized low-density lipoprotein（OX-LDL）.Methods Human monocytes purified by CD14＋ immuno-magnetic beads were differentiated and induced into immature DCs,which were randomly divided into 6 groups,Group A treated with PBS,Group B treated with OX-LDL,Group C and D treated respectively with GRb1 and ciglitazone,Group E and F were pretreated with the two testing drugs respectively followed by OX-LDL.The immuno-phenotypic expression（CD40,CD1a,and HLA-DR） and endocytosis function of DCs were examined using flow cytometry,the concentration of interleukin-12（IL-12） and tumor necrosis factor α（TNF-α） in the culture supernatants were measured with ELISA.Results Compared with Group B,Group E showed significantly lowered immuno-phenotypic expression of DCs in terms of CD40（67.4±1.62 vs.145.69±14.86）,CD1a（79.64±3.04 vs.159.89±6.09）,and HLA-DR（46.43±2.85 vs.99.33±17.11）,as well as higher endocytosis level（88.13%±1.06% vs.25.90%±5.77%,all P0.01）.Meantime,the serum levels of IL-12（88.65±5.59 ng/L vs.716.69±36.35 ng/L） and TNF-α（133.27±11.98 ng/L vs.968.10±36.42 ng/L） obviously decreased（P0.01）.The surface molecular expression of DCs and the secretion of inflammatory factors in Group F also obviously decreased,showing insignificant difference from Group E（P0.05）.Conclusion GRb1 could obviously inhibit the OX-LDL-induced maturation of DCs,showing similar effects to ciglitazone.