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复方白龙片对人胃癌细胞两条信号通路的调控
引用本文:谷善青,梁云燕,樊立人,李宝元,王代树.复方白龙片对人胃癌细胞两条信号通路的调控[J].中国中西医结合杂志,1997,17(7):404-407.
作者姓名:谷善青  梁云燕  樊立人  李宝元  王代树
作者单位:北京医科大学临床肿瘤学院
摘    要:目的:观察复方白龙片,对人胃癌细胞内两条信号通共调作用。方法:分别测定不同状况下人胃癌MGc80-3细胞内cAMP水平、二酰基甘油(DAG)含量、蛋白激酶A(PKA)和蛋白激酶C(PKC)活性。结果白龙处理MGc80-3细胞3h后,cAMP水平和PKA活性分别较对照组升高112.7%和19.7%,而DAG含量与PKC活性分别下降47.0%和64.2%;如用GF-109203X阻断PKC通路时发现c

关 键 词:复方白龙片  信号系统  胃肿瘤  中医药疗法

Regulation of Traditional Chinese Medicine Formulae Bailong on cAMP-PKA and DAG-PKC Signal Pathways of Human Gastric Cancer Cells
Authors:S Q Gu  Y Y Liang  L R Fan
Affiliation:Laboratory of Cell biology, Oncology School of Beijing Medical University.
Abstract:OBJECTIVE: To evaluate the effects of traditional Chinese medicine formulae (Bailong) on cAMP/PKA and diacylaglycerol (DAG) protein kinase (PKC) pathways of the MGc80-3 cells. METHODS: cAMP level, DAG content and activities of PKA and PKC were measured in different groups: (1) control, (2) 1.8 mg/ml Bailong, (3) 1.8 mg/ml Bailong +20 ug/ml PKA inhibitor, (4) 5 mumol/L PKC inhibitor. RESULTS: When MGc80-3 cells were in treatment with Bailong for 3 hours, cAMP level and PKA activity were 11.27%, and 19.7% higher than that of the control, while DAG content and PKC activity were 47.0% and 64.2% lower than that of the control; When PKC pathway was blocked by PKC inhibitor GF-109203X, cAMP level and PKA activity were increased by 78.8% and 33.5% as against that of the control, while the DAG content and PKC activity were decreased by 40. 3% and 56.3% respectively. When MGc80-3cells were treated with Bailong, and at the same time, blocked PKA pathways by PKA inhibitor, cAMP level and PKA activity were decreased by 46.0% and 28.9%, on the other hand, DAG content and PKC activity were increased by 50.7% and 51.6% as against that of the Bailong group. CONCLUSIONS: (1) There was relationship of causes and result between differentiation of MGc80-3 cells and the signal pathways. (2) Results of this study were similar to that of hexamethylenebisacetamide (HMBA). It is shown that the two signal systems are the foundation of regulative effects of Chinese medicine formulae Bailong or HMBA on proliferation and differentiation in MGc80-3 cells.
Keywords:traditional Chinese formula Bailong  signal system  gastric cancer
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