基于聚类分析和典型相关分析的北柴胡保肝作用谱效关系研究

目的 探讨北柴胡正丁醇萃取部位的保肝作用及与其HPLC指纹图谱间的谱效关系，以阐明其药效物质基础。方法 采用HPLC-ELSD法获取北柴胡正丁醇萃取部位的指纹图谱；检测CCl₄所致急性肝损伤小鼠血清中丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）水平、肝组织中丙二醛（MDA）的量和超氧化物歧化酶（SOD）的活性；利用聚类分析与典型相关分析法对色谱-药效数据进行相关分析。结果 从HPLC指纹图谱中共提取出12个能够标示药材特征的皂苷类成分的共有峰。不同产地北柴胡药材的保肝作用存在显著差异（P＜0.05）。聚类分析和典型相关分析明确了给予北柴胡正丁醇萃取部位的小鼠血清中ALT水平和肝组织中SOD活性与皂苷类化合物柴胡皂苷a和柴胡皂苷d之间存在线性关系。结论 北柴胡保肝作用的部分药效物质基础为柴胡皂苷a和柴胡皂苷d。

Spectrum-effect relationship of Bupleurum chinense for hepatoprotective effect based on cluster analysis and typical correlation analysis

Objective To analyze the spectrum-effect relationship between the hepatoprotective effect and HPLC fingerprints of the n-butanol fraction extracted from Bupleurum chinense and to elucidate its effective substance. Methods HPLC-ELSD was used to obtain the fingerprint peaks of n-butanol fraction of B. chinense; the levels of alanine transarninase (ALT) and aspartate aminotransferase (AST) in serum, malondialdehyde (MDA) content, and superoxide dismutase (SOD) activity in liver tissue of mice with acute hepatic injury induced by CCl₄ were detected. Cluster analysis and typical correlation analysis were used to analyze the data of spectrum and efficacy. Results Twelve common peaks which could show the features of B. chinense were isolated and there was statistical difference (P < 0.05) among the hepatoprotective effects of B. chinense from different origins. Saikoside a and saikoside d were proved to have linear relationship with the serum ALT level and liver tissue SOD activity in mice with acute liver injury in the treatment group by cluster analysis and typical correlation analysis. Conclusion Saikoside a and saikoside d are the effective substances of B. chinense with hepatoprotective effect.