败酱草及其混伪品的显微鉴别特征与HPLC指纹图谱研究

目的 对败酱草Herba Patriniae及其市场常见混伪品的显微鉴别特征和HPLC指纹图谱进行研究，明确不同品种的败酱草及其混伪品间的差异，为败酱草的准确鉴定提供技术保障。方法 收集败酱草正品药材黄花败酱和白花败酱，市场常见败酱草混用品异叶败酱、菥蓂和苣荬菜，采用显微鉴别方法对5种药材粉末的显微特征进行研究；优化败酱草总皂苷的提取工艺，比较不同品种的总皂苷含量；建立败酱草及其混伪品的HPLC指纹图谱，基于指纹图谱相似度评价、聚类分析和主成分分析，对败酱草及其混伪品进行鉴别和药材质量的综合评价。结果 败酱草及其混伪品药材粉末的显微特征存在显著差异；优化了败酱草总皂苷的提取工艺，结果表明，败酱草及其混伪品总皂苷含量存在差异；建立了败酱草及其混伪品共26批的HPLC指纹图谱，共标定了11个共有峰，明确了败酱草及其混伪品指纹图谱的差异，通过相似度分析、聚类分析和主成分分析，可将败酱草及其混伪品进行准确区分；综合评价分析结果表明，败酱草及其混伪品的得分差异较大，其中正品白花败酱和黄花败酱的得分最高。结论 获得了败酱草及其市场常见混伪品在粉末的显微特征和HPLC指纹图谱的差异，为败酱草的准确鉴...

Research of microscopic identification characteristics and HPLC fingerprints of Herba Patriniae and its adulterants

Objective To investigate the microscopic identification characteristics, HPLC fingerprints of Baijiangcao (Herba Patriniae) and its common adulterants in the market, determine the typical characters between different varieties of Herba Patriniae and its adulterants, and provide technical support for the accurate identification of Herba Patriniae. Methods The genuine medicinal materials of Herba Patriniae, Patrinia scabiosaefolia and P. villosa, as well as its common adulterants of Herba Patriniae, including Patrinia heterophylla, Thlaspi arvense and Sonchus arvensis were collected from the herbal medicine market. The microscopic characteristics of five medicinal powders were identified and analyzed through typical microscopic identification method. The the contents of total saponins from different varieties were determined with the optimized extraction appraoch of total saponins from P. scabiosaefolia. The combined strategy based on HPLC fingerprints, similarity analysis, cluster analysis and principal component analysis was performed to comprehensively evaluate and analyze the medicinal quality of Herba Patriniae and its adulterants. Results There was a significant difference between Herba Patriniae and its adulterants in the microscopic characteristic. The optimized extraction appraoch of total saponins from P. scabiosaefolia was determined. The total saponins contents from Herba Patriniae and its adulterants were different. The fingerprints of Herba Patriniae and its adulterants were established, and 11 common peaks were identified. There were significant differences in fingerprints of different varieties. Based on similarity analysis, cluster analysis and principal component analysis, Herba Patriniae and its adulterants can be distinguished. The results of comprehensive evaluation and analysis showed that the scores of Herba Patriniae and its adulterants are quite different, among which genuine Patrinia villosa and P. scabiosaefolia had the highest scores. Conclusion In this study, the differences of powder microscopic characteristics and HPLC fingerprints among Herba Patriniae and its adulterants was obtained, which provided technical support for accurate identification of Herba Patriniae and further clinical medication safety. These results would also contribute to the resource utilization and development of potential medicinal value of the market adulterants of Herba Patriniae.