大孔树脂纯化芦笋总皂苷的工艺研究

目的研究芦笋总皂苷的大孔树脂纯化工艺。方法采用静态及动态吸附解吸试验优选适宜的大孔树脂,并优化纯化条件。结果 S-8型树脂对芦笋总皂苷有较好的吸附和洗脱效果,并具有良好的脱色效果,其最佳纯化条件为上样质量浓度1 mg/mL,上样量60 mL/g树脂,用pH 7的75%乙醇洗脱,洗脱剂用量为10 mL/g树脂。纯化后得提取物中芦笋总皂苷的质量分数可达50.15%,脱色率为92.23%。结论 S-8型大孔树脂用于富集芦笋总皂苷效果最佳,是一种理想的分离纯化介质。

Purification of total saponins from Asparagus officinalis by macroporous resin

Objective To investigate the purification of saponins from Asparagus officinalis by macroporous resin.Methods Macroporous resin was chosen with static and dynamic adsorption and desorption experiments to optimize the purification parameters.Results S-8 macroporous resin was found to have good adsorption and desorption effects and show an excellent decolor effect.The optimum purification conditions were 1 mg/mL of saponins eluted by 75% ethanol at pH value 7 with a volume of 10 mL/g,and the loaded amount was 60 mL/g.The purity of total saponons increased to 50.15% after the purification,and the decoloration rate maintained 92.23%.Conclusion S-8 is an ideal resin with the best enrichment for separating and purifying total saponins from A.officinalis.