稳定性冠心病血瘀证的血浆定量蛋白质组学探析＊

目的 基于数据非依赖性采集的定量蛋白质组学技术（data independent acquisition,DIA）对稳定性冠心病血瘀证患者的血浆蛋白质进行研究分析,初步探索冠心病血瘀证的关键生物学过程与核心靶点。方法 选择中国中医科学院广安门医院心血管科收治的5例稳定性冠心病患者作为疾病观察组,同时选取健康成人5人作为对照组,采用DIA蛋白质组学技术对受试者的血浆进行检测,确定蛋白质表达量后进行差异蛋白质表达分析、GO富集分析、KEGG信号通路图查询和蛋白质互作网络分析。结果 在差异倍数为1.5倍时,疾病观察组相比于健康对照组,共鉴定到22个蛋白质下调,9个蛋白质上调。进一步的生物信息学分析表明,冠心病血瘀证的生物学过程与补体参与的慢性炎症反应有关,关键信号通路是补体与凝血级联反应的信号通路,核心蛋白质为C反应蛋白、转甲状腺素蛋白、补体因子H、维生素D结合蛋白等。结论 冠心病血瘀证的生物学过程与补体参与的慢性炎症反应有关。

Quantitative Proteomics Analysis on Blood Stasis Syndrome of Stable Coronary Artery Disease

Objective Based on the quantitative proteomics of data independent acquisition (DIA) to analyze the plasma proteins of patients with blood stasis syndrome of coronary artery disease (CAD), and explore the key biological process and potential biomarkers. Method Five patients with stable CAD from the department of cardiology, Guang''anmen Hospital, were selected as the observation group, and five healthy adults were selected as the control group. The plasma of the subjects was detected by DIA proteomics technology. After determining the differential protein, we perform differential protein expression analysis, GO enrichment analysis, KEGG signal pathway map query and protein-protein interaction analysis.Results When the fold change was 1.5, compared with the control group, 22 proteins in the observation group were down-regulated and 9 proteins were up-regulated. Further bioinformatics analysis showed that the biological process of blood stasis syndrome of SCAD is related to the chronic inflammatory response in which the complement system is involved. The core signal pathway is the complement and coagulation cascade. The core targets are von Willebrand factor, complement factor H.Conclusion This study initially screened the candidate biomarkers for SCAD with blood stasis syndrome as complement factor H. The biological process of the blood stasis syndrome is related to the chronic inflammatory response in which the complement system is involved.