基于网络药理学探讨丹参通络方治疗冠心病血瘀证的机制＊

目的 基于网络药理学探讨丹参通络方治疗冠心病（Coronary heart disease，CHD）血瘀证的可能作用机制。方法 通过TCMSP数据库ChEMBL数据库筛选出丹参通络方3味药丹参、三七、银杏叶潜在活性成分及靶点，通过GeneCard数据库获得冠心病的作用靶点，结合课题组前期通过高通量测序方法获得的冠心病血瘀证的差异表达基因，最终得到药物与疾病的交集靶点，并运用Cytoscape 3.7.2软件绘制活性成分-靶点-疾病网络图，采用STRING数据库构建PPI网络，使用R软件和Bioconductor包对交集靶点进行基因GO功能和KEGG富集分析。结果 筛选得到丹参通络方治疗冠心病血瘀证的潜在活性成分8个，核心靶点7个，其作用的生物路径与IL-17信号通路等关系密切。结论 丹参通络方治疗冠心病血瘀证的作用具有多成分、多靶点、多通路的作用机制特点，网络药理学结果可为实验验证其机制提供方向。

Mechanism of Danshen Tongluo Formula in the Treatment of Coronary Heart Disease with Blood Stasis Syndrome Based on Network Pharmacology

Objective To explore the possible mechanism of Danshen Tongluo formula (DT) for treating coronary heart disease with blood stasis syndrome (CHDBSS) based on network pharmacology.Methods The potential active components and targets of Radix Salviae Miltiorrhizae, Radix Notoginseng and Folium Ginkgo were screened out from the TCMSP and ChEMBL database. Targets of coronary heart disease (CHD) were obtained through the GeneCard database. Combined with the differential genes which are expressed in CHDBSS acquired from the previous high-throughput sequencing-techniques of the research group, the intersection target of medicinals and diseases was finally obtained. Cytoscape 3.7.2 was used to draw the network map of active component-target-disease. PPI network was constructed by consulting STRING database, and Bioconductor was used to do the GO function and KEGG enrichment analysis of intersection targets.Results There were 8 potential active components and 7 core targets of DT for treating CHDBSS, and the biological pathway of its action was closely related to IL-17 signaling pathway.Conclusion The effect of DT on CHDBSS has the characteristics of multi-ingredient, multi-target and multi-path mechanism, and this study can provide a direction for experimental verification of this mechanism.