基于聚类分析和主成分分析的叶下珠多酚部位HPLC指纹图谱研究

目的：建立基于聚类分析和主成分分析的不同产地叶下珠多酚部位高效液相色谱（HPLC）指纹图谱分析方法。方法：采用HPLC法测定9个不同产地叶下珠多酚部位指纹图谱，对其进行相似度分析、聚类分析和主成分分析，高效液相色谱-质谱（HPLC-MS）指认主要成分。结果：叶下珠多酚部位指纹图谱检出7个共有峰，指认了其中5个共有峰，9个不同产地叶下珠多酚部位相似度达到0.972-0.998，聚类分析和主成分分析从化学成分上说明了9批样品的相似性及差异性。结论：以指纹图谱数据为基础，将聚类分析与主成分分析结合起来进行识别，为叶下珠药材的资源利用提供依据。

HPLC Fingerprint of Total Phenol in Phyllanthus urinaria L. from Different Habitats Based on Cluster Analysis Coupled with Principle Component Analysis

In this study, HPLC fingerprint of total phenol in Phyllanthus urinaria L. from different habitats was investigated based on the cluster analysis coupled with principle component analysis. HPLC was used in the determination of total phenol in P. urinaria L. from 9 different habitats. The main components were identif ied with the similarity analysis, cluster analysis, principal component analysis and HPLC-MS. The results showed that there were 7 common peaks in the fingerprint of total phenol in P. urinaria L. There were 5 identified common peaks. The similarity of total phenol in P. urinaria L. from 9 different habitats was between 0.972-0.998. The cluster analysis and principal component analysis showed the similarities and differences of chemical components among 9 batches of samples. It was concluded that on the basis of fingerprint data, the cluster analysis coupled with principle component analysis can provide evidences for the identification and further utilization of P.urinaria L. resource.