电针“水沟”穴对大脑中动脉梗死大鼠脑血管平滑肌蛋白激酶C的影响

目的:观察针刺"水沟"穴对大脑中动脉梗死(MCAO)大鼠蛋白激酶C(PKC)蛋白水平及活性的影响,探讨针刺"水沟"调节血管平滑肌收缩的分子机制。方法:Wistar大鼠随机分为模型组、针刺组、假手术组及空白组,每组分0.5h、1h、3h、6h和12h5个亚组。Longa线栓法复制MCAO模型。针刺组电针"水沟"穴,刺激20min。用免疫组化法检测PKC在血管平滑肌细胞表达水平,用ELISA法检测PKC活性。结果:PKC表达水平模型组较空白组增加(P0.05),各时相点针刺组均低于模型组(P0.05);模型组PKC相对活性值较空白组上升(P0.05),针刺组与模型组比较,PKC活性显著降低(P0.05)。结论:MCAO大鼠血管平滑肌PKC蛋白水平和活性增加,可能参与调节大脑中动脉平滑肌收缩。针刺MCAO大鼠"水沟"穴,下调PKC蛋白水平和活性,可能缓解大脑中动脉平滑肌痉挛。

Effect of Electroacupuncture at "Shuigou" (GV 26) on Immunoactivity and Content of Protein Kinase C in the Middle Cerebral Artery in Acute Cerebral Infarction Rats

Objective To observe the effect of electroacupuncture(EA)intervention on expression and content of protein kinase C(PKC)in the middle cerebral artery in acute cerebral infarction(ACI)rats so as to explore its mechanism underlying improvement of ACI.Methods Wistar rats were randomly divided into normal control(n=6),sham operation(n=30),ACI model(n=30),and EA(n=30)groups,and the latter three groups were further divided into 0.5h,1h,3h,6hand 12hsubgroups(n=6in each subgroup).The ACI model was established by occlusion of the middle cerebral artery(MCAO).EA(15Hz,1mA)was applied to"Shuigou"(GV 26)for 20 min.The PKC expression levels and activity in the vascular smooth muscle of the middle cerebral artery were detected using immunohistochemistry and ELISA,respectively.Results In comparison with the control group,the immunoactivity and activities of PKC in the middle cerebral artery tissue at 0.5h,1h,3h,6hand 12 hwere significantly increased in the model group(P<0.05).After EA intervention,the expression levels and activities of PKC at the 5time-points were markedly down-regulated in comparison with the model group at the same corresponding time-point(P<0.05).No significant changes of PKC expression and activity were found in the sham operation group(P>0.05).Conclusion EA intervention can up-regulate the immunoactivity and activity of PKC in the vascular smooth muscle of the middle cerebral artery in ACI rats,which may contribute to its effect in improving ACI by relieving arterial spasm.