头皮针对脑缺血再灌注大鼠缺血区脑组织bcl-2、caspase-3蛋白表达以及血液流变的影响

目的:探讨头皮针治疗缺血性脑卒中的作用机制。方法:48只SD大鼠随机分为假手术组、模型组、针刺组,每组16只。采用线栓法制备大鼠大脑中动脉缺血再灌注模型。针刺组取顶颞前斜线和顶颞后斜线,分别在造模后6、12、24h治疗,每次电针20min。运用免疫组化法观察再灌注24h后各组大鼠缺血区脑组织bcl-2、caspase-3蛋白的表达,全自动血流变仪测定血液流变各项指标表达情况。结果:脑缺血再灌注后大鼠海马回bcl-2和caspase-3蛋白表达明显升高(均P<0.05),全血黏度和红细胞聚集性明显升高(均P<0.05);针刺组和模型组比较,大鼠缺血区脑组织bcl-2表达水平增高,caspase-3表达水平降低(均P<0.05),针刺组大鼠全血黏度和红细胞聚集性明显低于模型组(均P<0.05)。结论:头皮针刺可以明显促进大鼠缺血区脑组织抑凋亡蛋白的表达,同时降低促凋亡蛋白的表达,改善血液流变性,这可能是针刺减轻脑缺血损伤的机制之一。

Effects of Electroacupuncture on Cerebral Bcl-2 and Caspase-3 Expression after Cerebral Ischemia Reperfusion in Rats

Objective To investigate the underlying mechanism of electroacupuncture(EA)in anti-apoptosis effects in cerebral ischemia-reperfusion injury(CI/RI)rats.Methods Forty-eight SD rats were randomized into sham-operation(sham),model and acupuncture groups with 16 cases in each.CI/RI model was established by occlusion of the middle cerebral artery for 2 h and reperfusion for 24 h.Neuronal apoptosis and expression levels of the cerebral bcl-2 and caspase-3 after administration of EA(MS 6,MS 7)were detected by immunohistochemistry Whole blood viscosity,erythrocyte deformability and erythrocyte agreeability in rats after local CI/RI were assayed by using Automatic Blood Rheometer.Results In comparison with control group,bcl-2 and caspase-3 immunoactivity of the ischemic cerebral tissue,high,medium and low shear rates,aggregation index and rigidity index of the erythrocytes in model group increased significantly(P＜0.05),while the erythrocyte deformity index reduced strikingly(P＜0.05).Compared with model group,bcl-2 immunoactivity and erythrocyte deformity of acupuncture group increased remarkably(P＜0.05),and caspase-3 immunoactivity,high,medium and low shear rates of the whole blood viscosity.and erythrocyte aggregation index and rigidity index decreased considerably after acupuncture(P＜0.05),suggesting an improvement of the erythrocyte deformability and agreeability after acupuncture.Conclusion Acupuncture can resist neuronal apoptosis during CI/RI,which may be closely associated with its effects in upregulating bcl-2 expression,downregulating caspase-3 expression Moreover,acupuncture can significantly improve the erythrocyte deformability and reduce blood viscosity and erythrecyte agreeability.