平衡针法对局灶性脑缺血大鼠血清TNF-α、sICAM-1的影响及其相关性的分析

目的 :通过研究缺血性脑卒中大鼠针刺前后的血清TNF α、sICAM 1水平变化 ,探讨缺血性脑损伤机制及平衡针法在缺血性损伤中对脑的保护作用。方法 :将 60只大鼠随机分为空白组、假手术组、模型组、治疗组 ,光化学法诱导局灶性脑缺血模型 ,酶联法 (ELISA)测量血清TNF α、sICAM 1值。结果 :血清TNF α、sICAM 1水平模型组较空白组明显上升 (P <0 .0 0 1) ,平衡针治疗组较模型组有所降低 (P <0 .0 5) ,血清TNF α与sICAM 1呈正相关 (r=0 .83 4,P <0 .0 5)。结论 :局灶性脑缺血大鼠血清TNF α、sICAM 1含量明显上升 ,二者呈正相关。平衡针法治疗可降低其TNF α、sICAM 1水平 ,从一定程度上抑制缺血性脑损伤。

Effect of Balancing Technique of Acupuncture on Serum TNF-α and sICAM-1 Levels and Their Correlation in Regional Cerebral Ischemia Rats

Objective: To observe the effect of balancing technique of acupuncture on serum TNF-α and soluble intercellular adhesion molecule-1 (sICAM-1) levels and on their correlation in cerebral ischemia rats for analyzing the underlying mechanism of acupuncture in preventing the cerebral tissues from ischemic injury. Methods: Sixty Wistar rats were randomly divided into control group, sham-operation group, model group and acupuncture group, with 15 cases in each group. Regional cerebral ischemia (CI) model was established by using photochemically initiated thrombosis vascular obstruction method. Serum TNF-α and sICAM-1 contents were detected with enzyme linked immunosorbent assay. “Piantan”-, “Jiantong”-, “Xitong”- “Tuntong”- and “Yaotong”-points on the healthy side were punctured and stimulated with reducing technique.Results: In CI rats of model group, serum TNF-α (2.54±0.58 ng/mL) and sICAM-1 (17.54±4.18 ng/mL) contents were significantly higher than those of normal control group (1.61±0.59 ng/mL, 11.36±3.49 ng/mL) and sham-operation group (1.77±0.61 ng/mL, 12.71±3.56 ng/mL); while in acupuncture group, TNF-α (2.16±0.53 ng/mL) and sICAM-1 (14.91±3.22 ng/mL) contents were significantly lower than those of model group (P<0.05), suggesting that acupuncture can significantly inhibit CI induced increase of serum TNF-α and sICAM-1, which may contribute to the effect of acupuncture in preventing cerebral tissues from ischemic injury. Statistical analysis indicated that there was a positive correlation between serum TNF-α and sICAM-1 contents (r= 0.834, P<0.05) in their changes before and after CI. Conclusion: Balancing technique of acupuncture can effectively suppress CI induced elevation of serum TNF-α and sICAM-1 and there is a positive correlation between TNF-α and sICAM-1 levels.