枇杷叶紫珠化学成分及其细胞毒活性和抗炎活性

目的研究枇杷叶紫珠Callicarpa kochiana的化学成分及其细胞毒活性和抗炎活性。方法枇杷叶紫珠90%丙酮提取物采用硅胶、Sephadex LH-20、MCI、ODS、重结晶、半制备HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用CCK8法、Griess法、ELISA法测定化合物4~5的细胞毒活性和抗炎活性。结果从中分离得到5个化合物,分别鉴定为3,7,3’,4’-tetramethyl-quercetin (1)、pachypodol (2)、14α-hydroxyisopimaric acid(3)、14α-hydroxy-7,15-isopimaradien-18-oic acid (4)、(16R)-16,17-dihydroxyphyllolladan-3-one (5)。化合物4在一定浓度范围内对7种肿瘤细胞具有不同程度的抑制作用,并可以减少RAW264. 7细胞NO、IL-10、MCP-1、TNF-α的释放量。结论化合物1~5均为首次从该植物中分离得到,化合物4具有细胞毒活性和抗炎活性。

Chemical constituents from Callicarpa kochiana and their cytotoxic activity and anti-inflammatory activity

AIM To study the chemical constituents from Callicarpa kochiana and their cytotoxic activity and anti-inflammatory activity. METHODS The 90% acetone extract from C. kochiana was isolated and purified by silica,Sephadex LH-20,MCI,ODS,recrystallization and semi-preparation HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data. The cytotoxic activity and anti-inflammatory activity of the compounds 4-5 were determined by CKK8,Griess and ELISA methods. RESULTS Five compounds were isolated and identified as 3,7,3’,4’-Tetramethyl-quercetin(1),pachypodol(2),14α-hydroxyisopimaric acid(3),14α-hydroxy-7,15-isopimaradien-18-oic acid(4),(16 R)-16,17-dihydroxyphyllolladan-3-one(5).Compound 4 showed different degrees of inhibitory action for the seven kinds of cancer cells and reduced the emission of NO、IL-10、MCP-1、TNF-α in RAW264. 7 cells. CONCLUSION Compounds 1-5 are isolated from this plant for the first time,and compound 4 has cytotoxic activity and anti-inflammatory activity.