四物汤传统饮片汤剂与配方颗粒汤剂有效成分比较

目的比较四物汤传统饮片汤剂与其配方颗粒汤剂有效成分的含有量差异。方法四物汤传统饮片汤剂与其配方颗粒汤剂溶液的分析采用依利特SinoChrom ODB?BP柱(4.6 mm×150 mm,5μm);流动相乙腈?0.1%磷酸溶液,梯度洗脱;体积流量0.8 mL/min;检测波长215 nm(没食子酸),230 nm(芍药苷),330 nm(绿原酸、咖啡酸、阿魏酸、毛蕊花糖苷);柱温30℃。结果6种成分在各自范围内线性关系良好(r≥0.9996),平均加样回收率96.81%~99.01%,RSD 0.81%~1.58%。结论该方法准确稳定,重复性好,可用于四物汤的质量控制。

Comparison of effective components in traditional Slice Decoction and formulated Granule Decoction of Siwu Decoction

AIM To compare the content differences of effective components between traditional Siwu Decoction and formulated Granule Decoction.METHODS The analyses of solution of traditional Siwu Decoction and formulated Granule Decoction were performed on a 30℃thermostatic Elite SinoChrom ODB?BP column(4.6 mm×150 mm,5μm),with the mobile phase comprising of acetonitrile-0.1%phosphoric acid flowing at 0.8 mL/min in a gradient elution manner,and the detection wavelengths were set at 215 nm(gallic acid)、230 nm(peoniflorin)and 330 nm(chlorogenic acid、caffeic acid、ferulic acid、verbascoside),respectively.RESULTS Six constituents showed good linear relationships within their own ranges(r>0.9996),whose average recoveries were 96.81%-99.01%,with the RSD of 0.81%-1.58%.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of Siwu Decoction.