补肾活血法中药（糖络通）防治糖尿病大鼠早期视网膜病变的实验研究

目的：研究补肾活血法中药（糖络通）对糖尿病大鼠早期视网膜病变的防治作用及其作用机制。方法：随机将33只正常雄性Wistar大鼠分为正常对照组（N）11只，糖尿病对照组（D）11只，糖络通治疗组（DT）11只，连续给药8周后观察大鼠体重、空腹血糖、FPG、糖化血红蛋白（HbA1c）及大鼠视网膜RAGE mRNA的表达水平。并通过光镜电镜观察大鼠视网膜组织病理形态学的变化。结果：实验研究表明，糖络通可降低糖尿病大鼠视网膜RAGE mRNA的表达水平，组间比较有差异性（P＜0.01）；ALT， AST，Cr，BUN等安全性指标未出现异常，组间比较无统计学差异（P＞0.05）。结论：光镜、电镜观察表明糖络通对糖尿病大鼠的视网膜具有保护作用，能抑制神经节细胞及内外核层细胞的减少，减轻毛细血管扩张及视网膜组织的水肿。说明糖络通能够在一定程度上减缓DM大鼠视网膜的病理变化，对早期DR具有良好防治作用。为中医临床防治糖尿病微血管病变的研究提供理论依据。

An experimental study on treating early retinopathy in diabetes rats with the Tangluo Tong

Objective： To study the mechanism and preventive and therapeutic effects of Tangluo Tong on early retinopathy in diabetes rats. Methods：33 male rats were randomly divided into three groups, the control group （11 cases）, the therapeutic group （11 cases） and the treatment group （11 cases）, with 8 weeks’ medicine; the weight, the level of FPG, HbAlc and the expression of retinal RAGE mRNA in diabetes rats, and the histopathological changes were observed. Results： Tangluo Tong could reduce the expression level of retinal RAGE mRNA in diabetes rats with statistically significant difference （P〈0.01）;safe indicators such as ALT, AST, Cr, BUN were normal, there was no significant difference among groups （P〉0.05）. Tangluo Tong has protective effects for retina in diabetes rats, could inhibit the reduction of the ganglion cells and the inner and outer nuclear layer cells, and improve telangiectasias and retinal organization edema. Conclusion：The results suggest Tangluo Tong could improve pathological changes of retina in DM rats, prevent and treat early DR. All above provide theory basis for prevention and treatment study on diabetic microangiopathy in TCM.