鸭公树子中生物碱类成分及抗炎镇痛活性研究

目的 研究鸭公树子中生物碱类化学成分及抗炎镇痛活性。方法 采用Sephadex LH-20柱色谱法、薄层色谱法及高效液相制备色谱法等，对鸭公树子中的化学成分进行分离；通过核磁共振氢谱（¹H-NMR）、核磁共振碳谱（¹³C-NMR）、质谱法（MS）等鉴定化合物结构；采用二甲苯致小鼠耳廓肿胀实验和小鼠扭体实验分别观察单体成分的抗炎、镇痛作用效果。结果 从鸭公树子三氯甲烷提取部位中分离得到9个生物碱类化合物，分别鉴定为樟苍碱（1）、N-甲基樟苍碱（2）、乌药碱（3）、波尔定碱（4）、新木姜子碱（5）、杏黄罂粟碱（6）、异紫堇定碱（7）、烟酰胺（8）、N-反式阿魏酰酪胺（9）。新木姜子碱能抑制二甲苯所致的小鼠耳肿胀（P<0.01），并能减少醋酸所致的小鼠扭体次数（P<0.01）。结论 所有化合物均为首次从鸭公树子中分离得到，新木姜子碱显示出明显的抗炎镇痛活性，可能是该植物的活性成分之一。

Alkaloids of Seeds of Neolitsea chuii and the Anti-inflammatory and Analgesic Effects

Objective To study the alkaloid constituents and the material basis of anti-inflammatory and analgesic effects of seeds of Neolitsea chuii.Methods The chemical constituents of seeds of N. chuii were isolated by Sephadex LH-20 column chromatography, silica gel thin layer chromatography, and preparative high-performance liquid chromatography (HPLC). The structures of the compounds were identified by ¹H nuclear magnetic resonance (¹H-NMR), ¹³C-NMR and mass chromatography (MS). The anti-inflammatory and the analgesic effects of the isolated components were observed by xylene-induced auricle swelling test in mice and acetic acid-induced writhing test in mice, respectively.Results Nine alkaloids were isolated and identified from the trichloromethane extract of seeds of N. chuii, which were laurotetanine (1), N-methyl laurotetanine (2), coclaurine (3), boldine (4), laurolitsine (5), armepavine (6), isocorydine (7), nicotinamide (8), and N-trans-feruloyltyramine (9). Laurolitsine inhibited the auricle swelling induced by xylene in mice (P<0.01), and also reduced the number of writhing in mice caused by acetic acid (P<0.01).Conclusions These compounds mentioned above were isolated from seeds of N. chuii for the first time. Laurolitsine showed significant anti-inflammatory and analgesic activities, which may be one of the active constituents of the plant.