慢性激怒应激对大鼠胃动力和脑肠肽的影响及柴胡舒肝散的干预作用

目的探讨慢性激怒应激对大鼠胃窦平滑肌动力及下丘脑和血浆促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)、瘦素、胃动素的影响,以及柴胡疏肝散的干预作用。方法将40只Wistar大鼠随机分为4组,每组10只,正常组不给任何刺激;模型组、柴胡舒肝散组、生理盐水组均给予夹尾刺激,每次30min,每天2次,连续刺激14天。柴胡舒肝散组、生理盐水组每次应激后分别立即灌胃2ml柴胡散27.5g/(kg.d)]及生理盐水,每天2次。14天后检测各组大鼠胃窦肌条收缩活动,下丘脑及血浆CRH、ACTH、瘦素和胃动素水平。结果与正常组比较,模型组大鼠离体胃窦肌条收缩频率和振幅明显减少(P<0.01),下丘脑CRH、瘦素及血浆CRH、ACTH、瘦素浓度均明显增高(P<0.01),而下丘脑与血浆胃动素浓度则显著下降(P<0.01)。与模型组比较,柴胡舒肝散组胃窦肌条收缩频率和振幅显著增加(P<0.01),下丘脑和血浆CRH、ACTH及瘦素浓度显著降低(P<0.01),而下丘脑和血浆胃动素浓度则明显上升(P<0.01)。结论柴胡舒肝散可增加慢性激怒应激大鼠胃窦平滑肌动力,改善脑肠肽水平。

Influence of Chronic Enraged Stress on Gastric Motility and Brain-Gut Peptides in Rats and the Interfering Effect of Chaihu Shugan Powder

Objective To explore the influence of chronic enraged stress on the motility of antral smooth muscles,hypothalamic and plasma corticotropin releasing horm one(CRH),adrenocorticotropic hormone(ACTH),leptin and motilin in rats and the interfering effect of Chaihu Shugan Powder Power of Chaihu(Radix Bupleuri) fo r soothing the liver].Methods Forty Wistar rats were randomiz ed into 4 groups,with 10 in each.The normal group was not given any stimulation.The rats in the other g roups were stimulated by nipping at the tails for 30min,twice daily.Chaihu Shugan Powder 27.5g/(kg·d)]or normal saline was added to the observati on group and the control group respectively through gastric feeding.After 14 da ys,the motility of antral smooth muscles,hypothalamic and plasms CRH,leptin and motilin were observed.Results The frequency and amplitude of the antral mus cle contraction in the mo del group was significantly lower than that in the normal group(P<0.01).T he le vel of hypothalamic CRH and leptin,and plasma CRH,ACTH and leptin were significantly increased in the model group(P<0.01),while the level of hypothalam ic an d plasma motilin significantly decreased(P<0.01).Comparing with the model grou p,the frequency and amplitude of the antral muscle contraction and the level of hypothalamic and plasm motilin was significantly increased(P<0.01),but t he level of hypothalamic and plasma CRH,ACTH and leptin decreased significantly(P <0.01).Conclusion Chaihu Shugan Powder could increas e the gastric motility and improve the level of bran-gut peptides,