大蒜素对三硝基苯磺酸诱导大鼠结肠炎的保护作用及其机制研究

背景：溃疡性结肠炎（UC）是一种病因未明的结直肠炎症，大蒜素对其防治的作用目前尚未有结论。目的：探讨大蒜素对TNBS诱导的大鼠结肠炎的保护作用及其机制。方法：80只大鼠随机分为对照组、TNBS组、大蒜素预防组、大蒜素灌胃组、大蒜素灌肠组、地塞米松组、柳氮磺吡啶组、巴柳氮钠组。以含150mg／kgTNBS的50％乙醇溶液灌肠制备大鼠结肠炎模型。造模2周后处死大鼠。行大体评分和病理学评分，以ELISA法测定血清TNF-α、IL-1β、IL-10、IL4含量，蛋白质印迹法检测NF—κB表达。结果：与对照组相比，TNBS组大体和病理学评分均明显升高（P〈0．05），体质量明显降低（P〈0．05），血清TNF-α、IL-1B含量显著升高（P〈0．05），血清IL-4、IL-10含量显著降低（P〈0．05），NF-κB表达明显升高（P〈0．05）；给予大蒜素预防或治疗后，上述指标均明显改善（P〈0．05），但疗效低于地塞米松组、柳氮磺吡啶组、巴柳氮钠组。结论：大蒜素对TNBS诱导的大鼠结肠炎有保护作用，可能是通过调控细胞因子和NF—κB而发挥作用的。

Protective Role and Mechanism of Diallyl Trisulfide in Trinitrobenzenesulfonic Acid-induced Colitis in Rats

Background:Ulcerative colitis(UC) is a disease of colorectal inflammation with unknown etiology,the protective effect of diallyl trisulfide(DATS) on UC is still controversial.Aims:To explore the protective role and mechanism of DATS in TNBS-induced colitis in rats.Methods:Eighty rats were randomly divided into 8 groups:control group,TNBS group,DATS prevention group,DATS gastric infusion group,DATS enteral infusion group,dexamethasone group, sulfasalazine group and balsalazide sodium group.Colitis model was established by infusion enema with 50%ethanol containing 150 mg/kg TNBS.All the rats were sacrificed 2 weeks later,macroscopic score and pathologic score were assessed.The serum levels of TNF-α,IL-1β,IL-10 and IL-4 were determined by ELISA.Expression of NF-κB in intestinal mucosa was detected by Western blotting.Results;Compared with control group,macroscopic score and pathologic score in TNBS group were significantly increased(P < 0.05 ),weight was significantly decreased(P < 0.05 ), serum levels of TNF-αand IL-1βwere significantly increased(P <0.05),serum levels of IL-4 and IL-10 were significantly decreased(P < 0.05 ),and expression of NF-κB was significantly increased(P < 0.05 ).When DATS was used for prevention or treatment of TNBS-induced colitis,all the above-mentioned indices significantly improved(P < 0.05 ), however,efficacy of DATS prevention or treatment was inferior to dexamethasone,sulfasalazine and balsalazide sodium. Conclusions:DATS plays a protective role in TNBS-induced colitis in rats,probably via the regulation of cytokines and NF-κB.