甲酰四氢叶酸钙调变5-氟脲嘧啶对胃癌原代细胞株增殖抑制效应 |
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引用本文: | 朱海杭,倪金良,李登銮,郭媛.甲酰四氢叶酸钙调变5-氟脲嘧啶对胃癌原代细胞株增殖抑制效应[J].胃肠病学,1999,4(2):86-88. |
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作者姓名: | 朱海杭 倪金良 李登銮 郭媛 |
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作者单位: | 江苏省苏北人民医院消化病研究室!扬州225001 |
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摘 要: | 探讨甲酰四氢叶酸钙(CF)调变5-氟脲嘧啶(5-FU)对不同胃癌原代细胞株的增殖抑制效应。方法:采用原代细胞培养和四唑氮蓝(MTT)药物敏感试验,观察大剂量CF(相当于临床用药300mg/d)和5-FU(相当于临床用药750mg/d)单剂和联合应用对胃癌原代细胞的体外抑制作用。结果:体外对胃癌原代细胞株直接抑制作用,单剂CF为11.82%.单剂5.FU为42.25%:两者联台应用为44.54%.仅43%的胃癌原代细胞株显示CF对5.FU有一定的增效作用。结论:单剂CF对胃癌原代细胞仅有轻度抑制作用,联合应用大剂量CF和5-FU仅显示CF对部分细胞株5-FU的抑制作用有一定的增强效应,个体差异性较大,为确保CF的生化调节作用,避免其大剂量使用时毒副作用.建议用药前行MTT法药敏试验,以指导临床制订合理联台应用CF和5-FU的化疗方案。
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关 键 词: | 胃癌 原代细胞 5-FU CF 增殖抑制 大剂量 5-氟脲嘧啶 效应 单剂 个体差异性 |
Regulatory Effect of CF on 5-FU Against Human Gastric Cancer Cell Line in Vitro |
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Abstract: | Background/Aims: To evaluate the regulatory effect of calcium folinate (CF) on 5-fluorouracil (5-FU) against human gastric cancer cell. Methods: Primary cell culture technique and MTT colorimetric assay were used to study propagational inhibition effect of CF, 5-FU and a combination of the two drugs on 30 fresh human gastric cancer cell lines in vitro. Results: The immediate propagational inhibition rates of CF, 5-FU and CF/5-FU against gastric cell lines were 11.82%, 42.25% and 44.54% respectively. The combination of the two drugs had a slight rise in propagational inhibition rate in 43% of gastric cancer cell lines. Conclusions: CF increased the anticancer effect of 5-FU in small part of gastric cancer cell lines in vitro. The drug sensitivity test by MTT should be performed before chemotherapy using CF and 5-FU. |
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Keywords: | Calcium folinate 5-Fluorouracil Combined chemotherapy MTT colorimetric assay Cell culture |
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