| 小檗碱抑制氧化型低密度脂蛋白诱导的人脐静脉内皮细胞增殖及其分子机制 |
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| 引用本文： | 马春艳,徐瑞霞,姚雨宏,李小林,郭远林,张彦,李莎,李建军.小檗碱抑制氧化型低密度脂蛋白诱导的人脐静脉内皮细胞增殖及其分子机制[J].中国动脉硬化杂志,2018,26(2):144-151. |
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| 作者姓名： | 马春艳 徐瑞霞 姚雨宏 李小林 郭远林 张彦 李莎 李建军 |
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| 作者单位： | 中国医学科学院北京协和医学院国家心血管病中心阜外医院心血管疾病国家重点实验室血脂异常与心血管病诊治中心; |
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| 基金项目： | 北京协和医学院协和青年基金项目(3332016018)；阜外医院科研人才支持计划项目(2012-FWXX02)；首都卫生发展科研专项项目(2016-1-4035) |
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| 摘 要： | 目的探讨小檗碱对氧化型低密度脂蛋白(ox-LDL)诱导人脐静脉内皮细胞(HUVEC)增殖的抑制作用及其相关信号通路改变。方法体外培养HUVEC,以ox-LDL刺激增殖并以不同浓度小檗碱干预,采用细胞计数试剂盒8与Ed U掺入实验检测小檗碱对HUVEC增殖的抑制作用,通过实时荧光定量PCR及Western blot分析相关基因和蛋白表达及信号通路变化。结果小檗碱(1~50 mg/L)呈浓度依赖性显著抑制ox-LDL诱导的HUVEC增殖,并降低增殖细胞核抗原(PCNA)、核因子кB(NF-κB)、凝集素样氧化型低密度脂蛋白受体1(LOX-1)的表达;该作用与PI3K/Akt、ERK1/2及p38MAPK信号通路蛋白的磷酸化水平降低相关。结论小檗碱通过下调PCNA、NF-κB与LOX-1表达从而抑制ox-LDL诱导的HUVEC增殖,PI3K/Akt、ERK1/2及p38MAPK信号通路参与其中。
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| 关 键 词： | 小檗碱 氧化型低密度脂蛋白 人脐静脉内皮细胞 细胞增殖 信号通路 |
| 收稿时间： | 2017/6/30 0:00:00 |
| 修稿时间： | 2017/8/20 0:00:00 |
Inhibitory effect of berberine on proliferation of human umbilical vein endothelial cells induced by oxidized low density lipoprotein and its molecular mechanism |
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| MA Chun-Yan,XU Rui-Xi,YAO Yu-Hong,LI Xiao-Lin,GUO Yuan-Lin,ZHANG Yan,LI Sha and LI Jian-Jun.Inhibitory effect of berberine on proliferation of human umbilical vein endothelial cells induced by oxidized low density lipoprotein and its molecular mechanism[J].Chinese Journal of Arteriosclerosis,2018,26(2):144-151. |
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| Authors: | MA Chun-Yan XU Rui-Xi YAO Yu-Hong LI Xiao-Lin GUO Yuan-Lin ZHANG Yan LI Sha and LI Jian-Jun |
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| Affiliation: | Diagnosis and Treatment Center for Dyslipidemia and Cardiovascular Diseases, State Key Laboratory of Cardiovascular Diseases, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100037, China,Diagnosis and Treatment Center for Dyslipidemia and Cardiovascular Diseases, State Key Laboratory of Cardiovascular Diseases, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100037, China,Diagnosis and Treatment Center for Dyslipidemia and Cardiovascular Diseases, State Key Laboratory of Cardiovascular Diseases, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100037, China,Diagnosis and Treatment Center for Dyslipidemia and Cardiovascular Diseases, State Key Laboratory of Cardiovascular Diseases, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100037, China,Diagnosis and Treatment Center for Dyslipidemia and Cardiovascular Diseases, State Key Laboratory of Cardiovascular Diseases, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100037, China,Diagnosis and Treatment Center for Dyslipidemia and Cardiovascular Diseases, State Key Laboratory of Cardiovascular Diseases, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100037, China,Diagnosis and Treatment Center for Dyslipidemia and Cardiovascular Diseases, State Key Laboratory of Cardiovascular Diseases, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100037, China and Diagnosis and Treatment Center for Dyslipidemia and Cardiovascular Diseases, State Key Laboratory of Cardiovascular Diseases, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100037, China |
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| Abstract: | |
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| Keywords: | Berberine Oxidized low density lipoprotein Human umbilical vein endothelial cell Cell proliferation Signaling pathway |
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