川崎病患儿连接蛋白37基因多态性分析

目的研究连接蛋白37(Cx37)基因C1019T多态性与川崎病发病及并发冠状动脉损伤(CAL)之间的关系。方法应用聚合酶链反应-限制性片长多态性分析技术结合琼脂糖凝胶电泳技术,检测45例川崎病患儿和60例正常对照组儿童Cx37基因C1019T多态性位点的基因型分布和等位基因频率。结果川崎病组Cx37基因C1019T多态性位点CC、TC、TT基因型分布和C、T等位基因频率与正常对照组比较差异无显著性意义(χ2=1.411和0.003,P>0.05)。川崎病患者中合并CAL组与无CAL组基因型分布和等位基因频率比较差异亦无显著性意义(χ2=0.633和0.438,P>0.05)。结论 Cx37基因C1019T多态性与川崎病及其CAL的发生均无明显相关性。

Investigation of Connexin 37 Gene Polymorphisms in Patients with Kawasaki Disease

Aim To investigate the association between the connexin37(Cx37) gene C1019T polymorphisms and the risk of Kawasaki disease(KD) and that complicated with coronary artery lesion(CAL).Methods A total of 45 patients with KD and 60 healthy controls were genotyped by using polymerase chain reaction-restriction fragment length polymorphism and agarose gel electrophoresis for the C1019T polymorphism in Cx37 gene.Results For C1019T polymorphism in Cx37 gene,there were no significant differences between KD patients and the controls in genotype frequencies of CC,TC and TT and allele frequencies of C and T(χ2=1.411 and 0.003,both P>0.05);there were no significant differences between KD patients with CAL and that without CAL in genotype and allele frequencies(χ2=0.633 and 0.438,both P>0.05).ConclusionNo association was found between C1019T polymorphism in Cx37 gene and the risk of KD or its complication of CAL in this study