胃癌组织H-ras基因点突变与预后的关系

目的:胃癌发生发展的分子基础仍不甚明了,为了明确H—ras点突变在胃癌发生发展中的作用,本研究对胃癌组织H—ras点突变进行检测。方法:采用多聚酶链延伸反应—限制性片段长度多态性分析法(PCR—RFLP)对88例福尔马林液固定、石蜡包埋胃癌组织H—ras第12位和61位密码子点突变作了检测,并对点突变与肿瘤生物学行为及预后的关系进行分析。结果:H—ras总突变率为14.8％(13/88),点突变的发生与肿瘤将膜浸润,淋巴结转移、临床分期及术后生存期密切相关。结论:检测胃癌组织H—ras基因点突变有助于判断胃癌患者的预后。

Study on the relation between H - ras gene point mutation and the prognosis of patients with gastric cancer

AIM The molecular basis of gastric cancer development is poorly understood.In order to evalue the role of H-ras mutation in theprogression of gastric cancer,we examined H-ras point mutation in gastric cancer.METHODS Polymerase chain reaction and restrication fragment length polymorphisms(PCR-RFLP)analysis was performed on88 formalin-fixed,paraffin-embedded gastric cancer tissues to determine the point mutation at codon12 and 61 of H-ras,gene.The relation between H-ras gene point mutation and the tumor biology and prognosis was analysed.RESULTS Point mutation was detected in 13/88(14.8%)of gastric cancer and the positivity of H-ras point mutation was related to the status of serosal invasion,lymph-node metastasis,stages and posturgical survival period.CONCLUSION Determination of H-ras point mutation in gastric cancer may be useful in predicting prognosis of patients with gastric cancer.