百部总碱对钉螺酯酶同工酶活性及糖原含量的影响

目的观察百部总碱对钉螺体内酯酶同工酶活性及糖原含量的影响,以探讨其杀螺机制。方法用浸杀72h的LC50浓度（6.5mg/L）百部总碱药液浸泡钉螺,于浸泡后12、24、48、72、96h挑选活螺取样,然后分别采用聚丙烯酰胺凝胶电泳技术和蒽酮比色法检测在浸泡过程中钉螺肝脏酯酶同工酶活性及软体组织糖原含量的变化情况。同时设无氯水浸泡组作为对照。结果在百部总碱浸泡的96h内,钉螺酯酶活性先升高,后降低,最后几乎完全丧失;体内糖原含量则随浸泡时间的延长而逐步降低,浸泡96h后较之对照组含量下降72.00%。结论百部总碱能够使钉螺体内的酯酶同工酶活性及糖原含量降低或减少。

Influences of stemona alkaloids on esterase isozymes activities and glycogen content in Oncomelania hupensis

Objective To observe the influences of stemona alkaloids on esterase isozymes activities and glycogen content of Oncomelania hupensis in order to explore the molluscicidal mechanism of stemona alkaloids. Methods O. hupensis snails were immersed in the liquid of stemona alkaloids at the concentration of 6.5 mg/L（72 h LC50）, the surviving ones were picked out and sampled after being immersed for 12, 24, 48, 72, 96 h, then PAGE and anthrone colorimetry methods were used to observe the changes of liver esterase isozymes activities and tissue glycogen content of O. hupensis during the immersion period. A group of snails immersed in de-chlorinous water served as control. Results Esterase isozymes activities firstly increased, and then decreased until almost lost completely during the 96 h immersion period. Meanwhile, glycogen content gradually decreased as the immersion time extended. After being immersed for 96 h, glycogen content decreased by 72. 00% compared with the control group. Conclusion Stemona alkaloids could inhibit the viability of O. hupensis by causing decrease of esterase isozymes activities and glycogen content.