| 日本血吸虫大陆株粘蛋白样蛋白部分基因的克隆 |
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| 引用本文： | 张愉快,刘彦,肖建华,廖力,刘传爱,王可耕.日本血吸虫大陆株粘蛋白样蛋白部分基因的克隆[J].热带病与寄生虫学,2004,2(1):16-18. |
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| 作者姓名： | 张愉快 刘彦 肖建华 廖力 刘传爱 王可耕 |
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| 作者单位： | 421001,湖南,衡阳,南华大学医学院寄生虫学教研室 |
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| 基金项目： | 湖南省教育厅科研基金资助(课题编号:4-02-JY-02-C388) |
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| 摘 要： | 目的构建日本血吸虫中国大陆株粘蛋白样蛋白(SjMLP)核酸疫苗。方法分子克隆常规操作将扩增产物 SjMLP 编码区基因序列克隆至载体 pUCm-T 中,然后亚克隆到真核表达载体 pcDNA3.1( )中。结果经酶切、PCR 及测序鉴定表明所构建的质粒 pUCm-T/SjMLP 和 pcDNA3.1( )/SjMLP 中含有所扩增的基因序列。结论成功地构建了含目的基因的真核表达质粒 pcDNA3.1( )/SjMLP,从而,为进一步对其进行 DNA 免疫系列研究工作奠定了基础。
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| 关 键 词： | 日本血吸虫 粘蛋白样蛋白 RT-PCR 克隆 |
| 修稿时间： | 2003年11月5日 |
Cloning of the gene coding for mucin-like protein from chinese Schistosoma japonicum |
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| Zhang Yukuai Liu Yan Xiao Janhua Liao Li Liu Chuan'ai Wang Kegeng.Cloning of the gene coding for mucin-like protein from chinese Schistosoma japonicum[J].Journal of Tropical Diseases and Parasitology,2004,2(1):16-18. |
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| Authors: | Zhang Yukuai Liu Yan Xiao Janhua Liao Li Liu Chuan'ai Wang Kegeng |
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| Affiliation: | Zhang Yukuai Liu Yan Xiao Janhua Liao Li Liu Chuan'ai Wang Kegeng Department of parasitology,school of medicine,Nanhua University,Hengyang City,HuNan,421001,China. |
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| Abstract: | Objective To clone the part gene coding for schistosoma japonicum mucin-like protein into the eukaryotic expression vector pcDNA3.1( )and to construct a DNA vaccine.Methods TThe fragment of the SjMLP was amplified by PCR and cloned into cloning vector pUCm-T,then it was subcloned into eukaryotic expression vector pcDNA3.1( ).Results The coding region of SjMLP gene was verified after tested by PCR and by sequencing.Conclusion The eukaryotic expression plasmid was successfully constructed,which pro- vided the basis for further study on DNA immunization. |
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| Keywords: | Schistosoma japonicum Mucin-like protein RT-PCR Cloning |
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