| 台湾蠛蠓rDNA-ITS序列测定与分析 |
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| 引用本文： | 陈敏,肖颖,林妙端,黄恩炯.台湾蠛蠓rDNA-ITS序列测定与分析[J].中国人兽共患病杂志,2018,34(8):728-732. |
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| 作者姓名： | 陈敏 肖颖 林妙端 黄恩炯 |
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| 作者单位： | 1.福建国际旅行卫生保健中心,福州 350001;2.宁德出入境检验检疫局,宁德 352100;3.三明出入境检验检疫局综合技术服务中心,三明 365000 |
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| 基金项目： | 国家质检总局科技计划项目(No. 2016IK034、2015IK036,福建省自然科学基金(No. 2013J01266)和福建省重点科技项目(No. 2014I0001)联合资助 |
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| 摘 要： | 目的 探讨rDNA-ITS基因序列在吸血蠓分子鉴定中的应用前景。方法 通过PCR扩增获取台湾蠛蠓rDNA-ITS基因,测定分析ITS1和ITS2基因序列,构建系统发育树。结果 台湾蠛蠓ITS1和ITS2片段长度分别为310 bp与360 bp,与汤斯维尔铗蠓的同源性最大,分别为71%和92%。系统发育分析显示,基于ITS1、ITS2基因序列的分子鉴定结果与传统形态学鉴定结果一致。结论 rDNA-ITS基因序列可作为蠓科昆虫分子鉴定的分子标记。
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| 关 键 词： | 台湾蠛蠓 rDNA ITS1 ITS2 |
| 收稿时间： | 2018-03-14 |
Determination and analysis of rDNA-ITS genes from Lasiohelea taiwana |
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| CHEN Min,XIAO Ying,LIN Miao-duan,HUANG En-jiong.Determination and analysis of rDNA-ITS genes from Lasiohelea taiwana[J].Chinese Journal of Zoonoses,2018,34(8):728-732. |
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| Authors: | CHEN Min XIAO Ying LIN Miao-duan HUANG En-jiong |
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| Affiliation: | 1.Fujian International Travel Healthcare Center, Fuzhou 350001, China;2.Ningde Entry-Exit Inspection and Quarantine Bureau, Ningde 352100, China;3.Comprehensive Technology Service Center of Sanming Entry-Exit Inspection and Quarantine Bureau, Sanming 365000, China |
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| Abstract: | The ITS1 and ITS2 genes sequences of Lasiohelea taiwana were amplified and determined to investigate the feasibility of rDNA-ITS in the molecular identification of blood-sucking midges. PCR was employed in the present study and phylogenetic tree was analyzed according to the ITS1 and ITS2 genes sequences. It was showed that the framents of ITS1 and ITS2 are 310 bp and 360 bp, respectively, sharing the greatest homology of 71% and 92% with Forcipomyia townsvillensis. It was demonstrated in the phylogenetic analysis that the molecular identification based on ITS1 and ITS2 gene sequences is consistent with the morphological identification. Therefore, the sequences of rDNA-ITS can be the molecular marker in the molecular identification of biting midges. |
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| Keywords: | Lasiohelea taiwana rDNA ITS1 ITS2 |
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