| LAMP技术在动物病毒病原检测中的应用进展 |
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| 引用本文： | 周广舟,徐苏丽,谭晓荣,陈小兵.LAMP技术在动物病毒病原检测中的应用进展[J].中国人兽共患病杂志,2013,29(2):179-182. |
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| 作者姓名： | 周广舟 徐苏丽 谭晓荣 陈小兵 |
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| 作者单位： | 1.河南工业大学生物工程学院,郑州 450001; Email:zhougzhou@gmail.com2.郑州大学附属肿瘤医院,郑州 450008 |
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| 基金项目： | 国家自然科学基金(No.31101931,No.81172240);河南工业大学科技创新人才培育计划(No.11CXRC13);河南工业大学博士启动专项基金(No.2010BS016)联合资助~~ |
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| 摘 要： | 环介导等温扩增技术(LAMP)是一种具有简单、快速、特异性强和扩增效率高的核酸检测技术,目前已在食品、卫生及疾病预防控制检验等领域得到了诸多应用。本文主要介绍了LAMP技术在实验及临床过程中检测肝炎病毒、流感病毒、HIV-1、狂犬病毒、乙脑病毒、冠状病毒、登革病毒等病毒病原感染的应用进展。
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| 关 键 词： | 核酸 环介导等温扩增 病毒 检测 |
| 收稿时间： | 2012-09-13 |
Progress in medicinal viruses detection by loop-mediated isothermal amplification technology |
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| ZHOU Guang-zhou,XU Su-li,TAN Xiao-rong,CHEN Xiao-bing.Progress in medicinal viruses detection by loop-mediated isothermal amplification technology[J].Chinese Journal of Zoonoses,2013,29(2):179-182. |
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| Authors: | ZHOU Guang-zhou XU Su-li TAN Xiao-rong CHEN Xiao-bing |
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| Affiliation: | 1. College of Bioengineering, Henan University of Technology, Zhengzhou 450001, China;2. The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China |
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| Abstract: | Loop-mediated isothermal amplification (LAMP) is a new and simple nucleic acid detection technology with high specificity and efficiency, which has been applied in diagnosis of food poisoning, hygiene issues and infectious diseases. This paper mainly focuses on the development of LAMP detection technology for medicinal viruses such as hepatitis virus, influenza virus, HIV-1, rabies virus, Japanese encephalitis virus, coronavirus, dengue virus and so on. |
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| Keywords: | nucleotides loop-mediated isothermal amplification virus detection |
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