感染日本血吸虫小鼠CD_4~ 和CD_8~ T细胞中Fas及FasL的表达研究

目的　探讨在日本血吸虫感染过程中 ,宿主 CD4 和 CD8 T细胞中死亡受体 (Fas)及死亡配体 (Fas ligand,FasL)的表达情况。　方法　利用免疫组化的方法检测在日本血吸虫感染过程中 ,Fas和 Fas L 在 CD4 和 CD8 T细胞中的表达。　结果　在日本血吸虫感染过程中 ,Fas和 Fas L在 CD4 和 CD8 T细胞的表达随感染时间的延长而升高 ,但感染10 wk后 ,Fas L在 CD4 T细胞的表达接近并超过 Fas的表达。　结论　在日本血吸虫感染过程中 ,CD4 T细胞凋亡比率超过 CD8 T细胞和 CD4 / CD8 T细胞比值下降与相关蛋白 Fas、Fas L的表达有关

STUDIES ON EXPRESSION OF FAS AND FAS L IN CD_4~ AND CD_8~ T CELL OF THE MICE INFECTED WITH SCHISTOSOMA JAPONICUM

Objective\ To study the expression of Fas and FasL in CD 4 and CD 8 T cell of the mice infected with Schistosoma japonicum . Methods\ Immunohistochemistry technique was used to detect the expression of Fas and Fas ligand (FasL) in CD 4 and CD 8 T cells. Results\ The expression of Fas and FasL increased in CD 4 and CD 8 T cells with prolonged infection, but the expression of Fas L in the CD 4 T cell closed with or exceeded that in CD 8 T cell after 10 weeks infection. Conclusion The higher apoptotic rate of CD 4 T and the lower ratio of CD 4 /CD 8 in the mice infected with S. japonicum were associated with the expression of Fas and Fas L.