瑞-姬氏染液复合染色法诊断卡氏肺孢子虫肺炎的研究

目的　制作颜色鲜明 ,结构清晰 ,易于观察的卡氏肺孢子虫包囊和滋养体染色标本。　方法　采用瑞 -姬氏染液复合染色法。　结果　染色后肺孢子虫包囊中的囊内小体有呈紫红色的细胞核和呈蓝色的细胞质 ,囊壁薄 ,淡蓝色 ,包囊各部分结构清晰可辨。滋养体有紫红色的细胞核和蓝色的细胞质。　结论　瑞 -姬氏染液复合染色法可在临床诊断和教学中推广应用

STUDY OF WRIGHT-GIEMSA'S COMPOUND STAIN FOR DIAGNOSIS OF PNEUMOCYSTIS CARINII PNEUMONIA

Objective To make bright color, clear and observable specimens of Pneumocystis carinii cyst and trophozoite. Methods The specimens were stained with Wright Giemsa's Compound Stain. Results After staining the amaranth cell nucleus and blue cytoplasm of the intracystic body in P. carinii cyst were observed. The light blue wall of the cyst was thin and the structure of the cyst was seen clearly. The nucleus and cytoplasm of the trophozoite was amaranth and blue respectively. Conclusion Wright Giemsa's Compound Stain may be an useful technique in clinical diagnosis and teaching.(For Fig. 1,2, to see inside back cover)