| 新疆水源性隐孢子虫的检测 |
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| 引用本文： | 孟静,赵媛媛,阿斯亚·拜山伯.新疆水源性隐孢子虫的检测[J].中国病原生物学杂志,2011(1). |
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| 作者姓名： | 孟静 赵媛媛 阿斯亚·拜山伯 |
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| 作者单位： | 山东省单县中心医院;新疆医科大学药学院药理学教研室; |
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| 基金项目： | 国家自然科学基金专项基金项目(No.30540026) |
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| 摘 要： | 目的检测新疆15个城市自来水和河水隐孢子虫污染情况。方法采集水样,分别应用改良美国环保局(EPA)1622法及巢式PCR(nested-PCR)方法进行检测。1)改良EPA1622法:水样经微孔滤膜抽滤、淘洗,磁抗体分离法分离纯化后免疫荧光染色鉴定。2)Nested-PCR法:用试剂盒提取纯化的隐孢子虫卵囊基因组DNA,针对隐孢子虫小亚单位核糖体RNA(18SrRNA)部分基因,依据文献设计并合成引物,用巢式PCR扩增,产物纯化后经SspⅠ及VspⅠ单酶切,并进行RFLP分析。结果2种方法检测新疆15个地区的自来水隐孢子虫卵囊均为阴性,改良EPA1622法检测乌鲁木齐市、昌吉市、伊宁市和吐鲁番市的河水隐孢子虫卵囊阳性。巢式PCR检测乌鲁木齐市和伊宁市水样,均扩增出约830 bp的特异片段,RFLP初步鉴定为小鼠隐孢子虫基因;昌吉市和吐鲁番市河水水样PCR检测阴性。结论新疆乌鲁木齐市、伊宁市河水检出隐孢子虫,初步鉴定为小鼠隐孢子虫。而当地的饮用水未受污染。
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| 关 键 词： | 水源性 隐孢子虫 巢式PCR PCR-RFLP |
Detection of Waterborne Cryptosporidium in Xinjiang |
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| MENG Jing,ZHAO Yuan-yuan,Asiya Baishanbo.Detection of Waterborne Cryptosporidium in Xinjiang[J].Journal of Pathogen Biology,2011(1). |
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| Authors: | MENG Jing ZHAO Yuan-yuan Asiya Baishanbo |
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| Affiliation: | MENG Jing1,ZHAO Yuan-yuan2,Asiya Baishanbo2 (Shanxian Central Hospital,Shanxian 274300,Shandong,China,2.Pharmacy Department of Xinjiang Medical University,Wulmqi 830054,China) |
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| Abstract: | Objective To detect Cryptosporidium contamination in tap water and rivers in fifteen cities of Xinjiang.Methods Water samples were collected from fifteen cities in Xinjiang.Samples were tested by a modified form of EPA method 1622 and nested PCR assay,respectively.The modified form of EPA method 1622 consists of several steps,such as filtration with a microporous membrane,elution,immunomagnetic separation,and immunofluorescence assay.Nested PCR was conducted as follows.First,genomic DNA of purified oocysts ... |
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| Keywords: | Waterborne Cryptosporidium nested PCR PCR-RFLP |
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