老年人血浆氧化还原状态的改变及意义

目的探讨低龄老年人、中年人和青年人组血浆氧化还原状态的改变及临床意义。方法选择河南省人民医院健康体检者100例,按年龄分三组：低龄老年组（n=35）,中年组（n=33）,青年组（n=32）。测定所有研究对象的血浆还原型谷胱甘肽（GSH）与氧化型谷胱甘肽（GSSG）,还原型辅酶Ⅱ（NADPH）与氧化型辅酶Ⅱ（NADP＋）,并计算GSH/GSSG,NADPH/NADP＋的氧化还原电位。结果随着年龄增长（从青年组到低龄老年组）,GSH,GSH/GSSG渐次减低,GSH/GSSG氧化还原电位渐次升高,向氧化方向明显偏移（P〈0.05）;NADPH,NADPH/NADP＋氧化还原态亦显示出与GSH,GSH/GSSG类似但较弱的变化。结论氧化还原态失衡、向氧化方向偏移与年龄增长有关,与衰老的发生发展存在某种紧密的内在联系。

Changes and significance of plasma redox status in the aged

Objective To discuss the changes and clinical significance of plasma redox status in younger older people,middle-aged persons and young people.Methods The health persons （n=100） were selected from People＇s Hospital of Henan Province and then divided into the younger older group （group A,n=35）,middle-aged group （group B,n=33） and young group （group C,n=32） according to their ages.The levels of plasma reduced glutathione hormone （GSH）,glutathione in oxidized form （GSSG）,reduced form of nicotinamide-adenine dinucleotide phosphate （NADPH） and oxidizing form of nicotinamide-adenine dinucleotide phosphate （NADP＋） were detected in all groups,and the redox potential of GSH/GSSG and NADPH/NADP＋ were calculated.Results GSH and GSH/GSSG decreased gradually,redox potential of GSH/GSSG increased gradually and offset toward oxidation direction （P0.05） as age increased （from group C to group A）.NADPH and NADPH/NADP＋ also showed the similar but milder changes.Conclusion The imbalance of redox status and offsetting toward oxidation direction are related to age increase,and have a close relationship with aging progress.