迷走神经性房颤大鼠模型的建立及心房电生理学研究

目的探索房颤(AF)模型发生维持机制的关键病理环节,建立稳定AF模型。方法选取健康雄性SD大鼠40只,将体质量均衡大鼠随机分为对照组、模型1组、模型2组及模型3组,每组10只(n=10)共4组,分别以不同浓度和天数尾静脉给予乙酰胆碱-氯化钙(ACh-CaCl2)溶液,进行造模条件优选。以Langendorff恒温恒流灌流系统,描记离体心电图,同时以S1S2程控刺激检测各组大鼠心房有效不应期(AERP)。运用Western blot方法检测AF模型大鼠心房肌Cav1. 2蛋白表达程度。结果大鼠AF造模不同条件成功率分别为:模型1组为100%,模型2组70%,模型3组0;大鼠不同造模条件35 d后,AF维持时间比较:模型1组与模型2组,模型1组与模型3组,模型1组与对照组,模型2组与对照组差异均显著(P <0. 05),大鼠造模后,各组AERP相比较,对照组>模型1组,模型2组>模型1组(P <0. 05);使用Western blot检测,模型组大鼠及对照组大鼠心房肌Cav1. 2表达量有变化。结论运用ACh(99μg/ml),Ca Cl2(10 mg/ml)这一给药浓度,持续尾静脉给药35 d,造模成功率较高,可诱发大鼠稳定AF模型。

Research on establishing vagal atrial fibrillation model and atrial electrophysiology

AIM AIM To investigate the crucial pathological process of atrial remodeling of atrial fibrillation (AF) and to establish a stable animal model of AF. METHODS Forty health rats were randomly divided into 4 equal groups (n=10, each): Control Group, Model 1 Group, Model 2 Group and Model 3 Group. The rats in different groups were given tail intravenous injections of ACh and CaCl₂ of different concentrations and days. A Langendorff system was used to obtain a rat atrial effective refractory period (AERP). Atrial protein was extracted from AF model rats and Cav1.2 protein was detected using Western blot. RESULTS The successful rates in different groups after 7 days were 100% in Model 1 Group, 70% in Model 2 Group and 0 in Model 3 Group. Significant differences were observed in the AF time after 35 days between Model 1 and Model 2 Groups, Model 1 and Model 3 Groups, Model 1 and Control Groups, and Model 2 and Control Groups (P≤0.05, all). AERP after 35 days was Control Group > Model 1 Group and Model 2 Group < Model 1 Group ( P≤0.05, both). There were differences in Cav1.2 between Control and Model Groups. CONCLUSION Tail intravenous injection of ACh 99 μg/ml CaCl2 10 mg/ml for 35 days achieves higher establishment of a stable atrial fibrillation model.