TGF-β对动脉钙化的影响

目的 :探讨转化生长因子 - β（TGF- β）在动脉钙化中的作用。方法 :建立体外动脉钙化的模型 ,培养钙化血管平滑肌细胞 ,在培养液中加入 TGF- β,观察钙沉积及细胞碱性磷酸酶的变化 ,同时观察钙化过程中骨间质蛋白骨钙素分泌情况。结果 :1μg/ L 的 TGF- β作用 10 d后 ,钙化细胞的钙沉积为 1.95± 0 .5 6 μm ol/ L,较钙化对照组 （1.71±0 .0 5 μm ol/ L）增加了 14.4% ,细胞碱性磷酸酶的活性为 5 46± 118IU/ L,比钙化对照组 （16 5± 6 3IU/ L）增加了2 95 % ,这种作用在第 8天达到高峰 ,以后略有下降。在钙化加重的同时 ,骨钙素分泌亦明显增加 ,达 0 .37± 0 .0 4ng/ L;而 TGF- β对非钙化的细胞无上述作用。结论 :1μg/ L 的 TGF- β可以促进钙化血管平滑肌细胞的钙化过程。

Transforming growth factor-

AIM:To investigate the role transforming growth factor-β (TGF-β) in the vascular calcification. METHODS:Using β-glycerin- p hosphate sodium salt to induce cultured bovine vessel smooth muscle cell to calc ify and this serves as a vascular calcifying model in vitro. TGF-β were add to the culture media. The deposition of calcium,alkaline phosphatase activity and osteocalcin concentration was quantified during the 10d culture processes. RESULTS:After 10 d of culture,1 μg/L TGF-β increased the calcium depo sition of calcified VSMC from 1.71±0.05 μmol/L to 1.95±0.56 μmol/L. The cell ular ALP activity was significantly increased from 165±63 IU/L of calcified con trol group to 546±118 IU/L with the maximum increase in the 8 d. The osteocal cin concentration of cultured media syntheses by BVSMCs was also elevated to 0.3 7±0.04 ng/L after TGF-β treatment. CONCLUSION:TGF-β increased cal cified BVSMCs calcium deposition via stimulating the cellular synthesis of ALP and osteocalcin and promoting the change of calcified BVSMC to osteoblastic cell .