乙酰化白藜芦醇通过调节缝隙连接蛋白43表达减轻海水淹溺型急性肺损伤

目的观察海水淹溺肺损伤大鼠肺组织中缝隙连接蛋白43（Cx43）的表达变化及乙酰化白藜芦醇对其的干预作用。方法32只大鼠完全随机分为空白对照组、模型组、乙酰化白藜芦醇预处理组和乙酰化白藜芦醇组，每组8只。采用气管内滴注海水（4ml／kg）的方法制作海水淹溺型急性肺损伤大鼠模型，用ELISA法检测肺组织中TNF-仅和IL-10的含量，实时定量PCR及免疫组化的方法检测肺组织中Cx43的表达变化。结果吸人海水4h后，大鼠肺部损伤明显，TNF—α和IL-10的含量增高，Cx43在基因水平表达量增高，而蛋白水平表达降低；乙酰化白藜芦醇预处理明显减轻海水吸入导致的急性肺损伤，减少炎症因子分泌并且在基因水平和蛋白水平增加Cx43的表达。结论Cx43参与海水淹溺型急性肺损伤的发生与发展，乙酰化白藜芦醇能够通过增加Cx43的表达减轻肺损伤。

Acetylated resveratrol alleviates seawater drowning induced acute lung injury by enhancing the expression of Connexin43

Objective To observe the expression of Connexin43 （Cx43） in rat lungs suffered from seawater inhalation and the intervention effect of acetylaled resveratrol. Methods 32 rats were randomly divided into normal control group, model group, acetylated resveratrol pre-treated group, and acetylated resveratrol group. Seawater drowning induced acute lung injury （SWD-ALI） model was made by instillation of seawater in to air way. Pathology detection was carried out on lungs four hours after modeling, ELISA was used to measure the contents of TNF-α and IL-10, and the expression of Cx43 in lungs was detected by using real- time PCR and immunohistochemistry. Results Obvious lung injury was detected 4 hours after seawater instillation, contents of TNF-α and IL-10 increased, and the expression of Cx43 was up-regulated on gene level but decreased on protein level. While pre-treatment of acetylated resveratrol attenuated lung injury, suppressed the secretion of inflammatory factors, and increased the expression of Cx43 on gene and protein level. Conclusions Cx43 plays part in occurrence and development of SWD-ALI, and acetylated resveratrol can attenuate ALI by enhancing the expression of Cx43.