表达视网膜Müller细胞的转基因小鼠基因鉴定△

目的 通过基因鉴定方法检测出可表达视网膜Müller细胞的Sox2-Cre和Rosa26转基因小鼠杂交的后代。方法 采用传统提取DNA、聚合酶链反应(PCR)扩增、电泳及免疫荧光方法检测。结果 PCR方法检测野生型小鼠是长度为324 bp的条带;杂合子小鼠是长度为250 bp和324 bp的双条带,且免疫荧光发现转基因小鼠的自发红色荧光可以和Müller细胞标记物谷氨酰胺合酶(GS)绿色荧光共标。结论 建立了可表达视网膜Müller细胞的转基因小鼠模型,为今后研究视网膜Müller细胞的增殖分化作用及其作用机制提供了动物模型。

Genetic identification of transgenic mice expressing retinal Müller cells

Objective To detect the hybrid offspring of Sox2-Cre and Rosa26 transgenic mice that can express retinal Müller cells by gene identification method. Methods The traditional DNA extraction, PCR amplification, electrophoresis and immunofluorescence methods were used for detection. Results The wild-type mice were detected by PCR as a 324 bp band, while the heterozygous mice was double bands of 250 bp and 324 bp. Moreover, immunofluorescence revealed that the spontaneous red fluorescence of transgenic mice could be co-labeled with the green fluorescence of Müller cells marker glutamine synthetase (GS). Conclusions Transgenic mice expressing retinal Müller cells were established to provide an animal model for further studying the proliferation and differentiation of retinal Müller cells and their mechanisms.