The effects of vascular endothelial growth factor (VEGF) on human orbital preadipocyte |
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| Authors: | Hee-young Choi Su-bong Name Ji-eun Lee Ik-soo Byon Je-hyun Seo |
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| Affiliation: | 1. Department of Ophthalmology, Pusan National University Hospital, Busan, South Korea;2. Medical Research Institute, Pusan National University, Busan, South Korea;3. Department of Plastic and Reconstructive Surgery, Pusan National University Yangsan Hospital, Yangsan, South Korea;4. Department of Ophthalmology, Pusan National University Yangsan Hospital, Yangsan, South Korea |
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| Abstract: | Purpose: To investigate the presence of the Vascular Endothelial Growth Factor (VEGF) and its receptor (VEGFR) in human orbital preadipocytes, and to evaluate the effect of VEGF on human orbital preadipocyte differentiation and adipogenesis in vitro.Results: Four isoforms of VEGF (VEGF121, 155, 189, and 206), VEGFR-1, VEGF-2, and neuropilin-1 were expressed in human orbital preadipocytes. Treatment with 100 ng/ml VEGF induced higher expressions of C/EBPα and LPL than the non-treated control (p = 0.03 and p = 0.01) or treatment with 50ng/ml (p = 0.04 for both). At both concentrations VEGF enhanced the accumulation of intra-cytoplasmic lipid versus the control, and treatment with 100 ng/ml VEGF induced more lipid accumulation than treatment with 50 ng/ml VEGF (p = 0.03).Conclusions: VEGF and VEGFR were observed in human orbital preadipocytes, and exogenous VEGF enhanced adipogenesis in these cells. These results suggest that VEGF plays a role as an autocrine or paracrine growth factor during human orbital preadipocyte differentiation. |
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| Keywords: | Adipogenesis orbital preadipocyte preadipocyte vascular endothelial growth factor (VEGF) vascular endothelial growth factor receptor (VEGFR) |
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