子宫内膜癌bcl-2癌基因的持续性表达及其临床意义

目的：研究子宫内膜癌ｂｃｌ－２癌基因的表达及其临床意义。方法：采用免疫组化ＡＢＣ法检测增生期、分泌期、单纯型增生、复合型增生及不典型增生子宫内膜共２６份，子宫内膜癌４９例的ｂｃｌ－２癌基因蛋白表达及雌、孕激素受体（ＥＲ、ＰＲ）的表达。结果：正常增生期子宫内膜、增生的子宫内膜存在ｂｃｌ－２的表达，与ＥＲ相关，分泌期子宫内膜ｂｃｌ－２表达下降；４９例子宫内膜癌中２６例ｂｃｌ－２表达阳性，占５３％，２９例ＥＲ表达阳性，占５９％，２５例ＰＲ表达阳性，占５１％。７２％ｂｃｌ－２表达阳性者ＥＲ阳性，７５％ｂｃｌ－２表达阴性者ＥＲ阴性（Ｐ＜０．０１）。６８％ｂｃｌ－２表达阳性者ＰＲ阳性，６２％ｂｃｌ－２阴性者ＰＲ阴性（Ｐ＜０．０５）。子宫内膜癌Ｇ１、Ｇ２级ｂｃｌ－２的表达率为６６％，显著高于Ｇ３级者（２１％）（Ｐ＜０．０５）。ｂｃｌ－２的表达与肌层浸润、手术分期无关，ｂｃｌ－２表达阳性及阴性者生存率统计差异无显著性。结论：子宫内膜ｂｃｌ－２的持续性表达与卵巢激素相互作用可能在子宫内膜癌发生、发展中起作用

Bcl-2 oncogene persistent expression in endometrial carcinoma and its clinical significance.

Objective:To determine the expression of bcl-2 in endometrial carcinoma and its relationship with clinicopathological features and prognosis.Methods:Expressions of bcl-2,ER,PR were determinated using immunohistochemical ABC method in paraffin embeded tissues in 49 women with endometrial carcinoma and 26 women with benign endometrium.Results:In benign endometrium,bcl-2 was present in glands of proliferative phase and hyperplastic endometrium,while a weak signal was detected in one of five secretory phase endometrium.Bcl-2 persistent expression was observed in majority(53%) of endometrial carcinoma.A significant correlation of bcl-2 immunoreactivity with estrogen receptor(P<0.01)and progesterone receptor status(P<0.05)were found.The bcl-2 persistent expression was found to be significantly higher in FIGO G1 and G2 than that in G3 tumors(P<0.05),while no significant difference was detected in tumors of different myometrial invasion,stages or patients' survival.Conclusion:bcl-2 persistent expression is highly correlated with the presence of hormone receptors and may be related to hormonal regulation in the genesis and development of endometrial carcinomas.